Obesity is induced in mice heterozygous for cyclooxygenase-2

John N. Fain, Leslie R. Ballou, Suleiman Bahouth

Research output: Contribution to journalArticle

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Abstract

In mice heterozygous for the cyclooxygenase-2 gene (COX-2±) the body weight was enhanced by 33% as compared to homozygous COX-2-/- mice. The weights of the gonadal fat pads in COX-2± mice were enhanced by 3.5 to 4.7 fold as compared to COX-2-/- mice and by 1.5 to 3.5 fold as compared to wild-type controls+/+. Serum leptin levels and leptin release by cultured adipose tissue of COX-2± mice were both elevated as compared to either control or COX-2-/- animals. The basal release of PGE2 or 6 keto PGF per fat pad over a 24 h incubation of adipose tissue was reduced by 80% and 95% respectively in tissue from COX-2-/- mice. NS-398, a specific COX-2 inhibitor, inhibited leptin release by 27% in adipose tissue from control mice, 31% in tissue from COX-1-/- mice and by 23% in tissue from COX-2± mice while having no effect on leptin release by adipose tissue from COX-2-/- mice. These data indicate that heterozygous COX-2 mice develop obesity which is not secondary to a defect in leptin release by adipose tissue.

Original languageEnglish (US)
Pages (from-to)199-209
Number of pages11
JournalProstaglandins and Other Lipid Mediators
Volume65
Issue number4
DOIs
StatePublished - Jul 3 2001

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Cyclooxygenase 2
Obesity
Genes
Adipose Tissue
Tissue
Leptin
Mouse Ptgs2 protein
Fats
Cyclooxygenase 2 Inhibitors
Dinoprostone
Animals
Body Weight
Weights and Measures
Defects

All Science Journal Classification (ASJC) codes

  • Physiology
  • Biochemistry
  • Pharmacology
  • Cell Biology

Cite this

Obesity is induced in mice heterozygous for cyclooxygenase-2. / Fain, John N.; Ballou, Leslie R.; Bahouth, Suleiman.

In: Prostaglandins and Other Lipid Mediators, Vol. 65, No. 4, 03.07.2001, p. 199-209.

Research output: Contribution to journalArticle

Fain, John N. ; Ballou, Leslie R. ; Bahouth, Suleiman. / Obesity is induced in mice heterozygous for cyclooxygenase-2. In: Prostaglandins and Other Lipid Mediators. 2001 ; Vol. 65, No. 4. pp. 199-209.
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