Observation of calcium-dependent unidirectional rotational motion in recombinant photosynthetic F1-ATPaSe molecules

Ward C. Tucker, Alon Schwarz, Tiferet Levine, Ziyun Du, Zippora Gromet-Elhanan, Mark L. Richter, Gilad Haran

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

ATP hydrolysis and synthesis by the F0F1-ATP synthase are coupled to proton translocation across the membrane in the presence of magnesium. Calcium is known, however, to disrupt this coupling in the photosynthetic enzyme in a unique way: it does not support ATP synthesis, and CaATP hydrolysis is decoupled from any proton translocation, but the membrane does not become leaky to protons. Understanding the molecular basis of these calcium-dependent effects can shed light on the as yet unclear mechanism of coupling between proton transport and rotational catalysis. We show here, using an actin filament γ-rotation assay, that CaATP is capable of sustaining rotational motion in a highly active hybrid photosynthetic F1-ATPase consisting of α and β subunits from Rhodospirillum rubrum and γ subunit from spinach chloroplasts (αR 3β R 3γC). The rotation was found to be similar to that induced by MgATP in Escherichia coli F1-ATPaSe molecules. Our results suggest a possible long range pathway that enables the bound CaATP to induce full rotational motion of γ but might block transmission of this rotational motion into proton translocation by the F 0 part of the ATP synthase.

Original languageEnglish (US)
Pages (from-to)47415-47418
Number of pages4
JournalJournal of Biological Chemistry
Volume279
Issue number46
DOIs
StatePublished - Nov 12 2004

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Adenosine Triphosphate
Observation
Calcium
Molecules
Protons
Hydrolysis
Rhodospirillum rubrum
Membranes
Proton-Translocating ATPases
Spinacia oleracea
Chloroplasts
Catalysis
Actin Cytoskeleton
Magnesium
Escherichia coli
Actins
Assays
Enzymes

All Science Journal Classification (ASJC) codes

  • Biochemistry

Cite this

Tucker, W. C., Schwarz, A., Levine, T., Du, Z., Gromet-Elhanan, Z., Richter, M. L., & Haran, G. (2004). Observation of calcium-dependent unidirectional rotational motion in recombinant photosynthetic F1-ATPaSe molecules. Journal of Biological Chemistry, 279(46), 47415-47418. https://doi.org/10.1074/jbc.C400269200

Observation of calcium-dependent unidirectional rotational motion in recombinant photosynthetic F1-ATPaSe molecules. / Tucker, Ward C.; Schwarz, Alon; Levine, Tiferet; Du, Ziyun; Gromet-Elhanan, Zippora; Richter, Mark L.; Haran, Gilad.

In: Journal of Biological Chemistry, Vol. 279, No. 46, 12.11.2004, p. 47415-47418.

Research output: Contribution to journalArticle

Tucker, WC, Schwarz, A, Levine, T, Du, Z, Gromet-Elhanan, Z, Richter, ML & Haran, G 2004, 'Observation of calcium-dependent unidirectional rotational motion in recombinant photosynthetic F1-ATPaSe molecules', Journal of Biological Chemistry, vol. 279, no. 46, pp. 47415-47418. https://doi.org/10.1074/jbc.C400269200
Tucker, Ward C. ; Schwarz, Alon ; Levine, Tiferet ; Du, Ziyun ; Gromet-Elhanan, Zippora ; Richter, Mark L. ; Haran, Gilad. / Observation of calcium-dependent unidirectional rotational motion in recombinant photosynthetic F1-ATPaSe molecules. In: Journal of Biological Chemistry. 2004 ; Vol. 279, No. 46. pp. 47415-47418.
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