Olfactory bulb muscarinic acetylcholine type 1 receptors are required for acquisition of olfactory fear learning

Jordan M. Ross, Mounir Bendahmane, Max Fletcher

Research output: Contribution to journalArticle

Abstract

The olfactory bulb (OB) receives significant cholinergic innervation and widely expresses cholinergic receptors. While acetylcholine (ACh) is essential for olfactory learning, the exact mechanisms by which ACh modulates olfactory learning and whether it is specifically required in the OB remains unknown. Using behavioral pharmacology and optogenetics, we investigated the role of OB ACh in a simple olfactory fear learning paradigm. We find that antagonizing muscarinic ACh receptors (mAChRs) in the OB during fear conditioning but not testing significantly reduces freezing to the conditioned odor, without altering olfactory abilities. Additionally, we demonstrate that m1 mAChRs, rather than m2, are required for acquisition of olfactory fear. Finally, using mice expressing channelrhodopsin in cholinergic neurons, we show that stimulating ACh release specifically in the OB during odor-shock pairing can strengthen olfactory fear learning. Together these results define a role for ACh in olfactory associative learning and OB glomerular plasticity.

Original languageEnglish (US)
Article number164
JournalFrontiers in Behavioral Neuroscience
Volume13
DOIs
StatePublished - Jul 9 2019

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Olfactory Bulb
Muscarinic Receptors
Fear
Acetylcholine
Learning
Cholinergic Receptors
Optogenetics
Cholinergic Neurons
Aptitude
Cholinergic Agents
Freezing
Shock
Pharmacology

All Science Journal Classification (ASJC) codes

  • Neuropsychology and Physiological Psychology
  • Cognitive Neuroscience
  • Behavioral Neuroscience

Cite this

Olfactory bulb muscarinic acetylcholine type 1 receptors are required for acquisition of olfactory fear learning. / Ross, Jordan M.; Bendahmane, Mounir; Fletcher, Max.

In: Frontiers in Behavioral Neuroscience, Vol. 13, 164, 09.07.2019.

Research output: Contribution to journalArticle

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