Olfactory nerve stimulation activates rat mitral cells via NMDA and non-NMDA receptors in vitro

Matthew Ennis, Lee A. Zimmer, Michael T. Shipley

Research output: Contribution to journalArticle

105 Citations (Scopus)

Abstract

THE neurotransmitter(s) and receptors mediating excitatory transmission at the mammalian olfactory nerve-mitral cell synapse were investigated using extracellular recordings in rat olfactory bulb slices. Single shocks applied to the olfactory nerve elicited both a short latency and a delayed excitatory response in mitral cells. Both responses were blocked after bath application of kynurenic acid, a broad-spectrum glutamate receptor antagonist, or DNQX, a preferential non-NMDA receptor antagonist. The specific NMDA receptor antagonist AP5 selectively attenuated the delayed, but not the initial excitation. These results suggest that glutamate is the major excitatory transmitter in the mammalian olfactory nerve, and excites mitral cells via NMDA and non-NMDA receptors.

Original languageEnglish (US)
Pages (from-to)989-992
Number of pages4
JournalNeuroReport
Volume7
Issue number5
DOIs
StatePublished - Jan 1 1996
Externally publishedYes

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Olfactory Nerve
N-Methylaspartate
Kynurenic Acid
Excitatory Amino Acid Antagonists
Neurotransmitter Receptor
Olfactory Bulb
N-Methyl-D-Aspartate Receptors
Baths
Synapses
Glutamic Acid
Shock
Neurons
In Vitro Techniques

All Science Journal Classification (ASJC) codes

  • Neuroscience(all)

Cite this

Olfactory nerve stimulation activates rat mitral cells via NMDA and non-NMDA receptors in vitro. / Ennis, Matthew; Zimmer, Lee A.; Shipley, Michael T.

In: NeuroReport, Vol. 7, No. 5, 01.01.1996, p. 989-992.

Research output: Contribution to journalArticle

Ennis, Matthew ; Zimmer, Lee A. ; Shipley, Michael T. / Olfactory nerve stimulation activates rat mitral cells via NMDA and non-NMDA receptors in vitro. In: NeuroReport. 1996 ; Vol. 7, No. 5. pp. 989-992.
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