On the expansion of ribosomal proteins and RNAs in eukaryotes

Michael S. Parker, Renu Sah, Ambikaipakan Balasubramaniam, Floyd R. Sallee, Edwards Park, Steven L. Parker

Research output: Contribution to journalReview article

4 Citations (Scopus)

Abstract

While the ribosome constitution is similar in all biota, there is a considerable increase in size of both ribosomal proteins (RPs) and RNAs in eukaryotes as compared to archaea and bacteria. This is pronounced in the large (60S) ribosomal subunit (LSU). In addition to enlargement (apparently maximized already in lower eukarya), the RP changes include increases in fraction, segregation and clustering of basic residues, and decrease in hydrophobicity. The acidic fraction is lower in eukaryote as compared to prokaryote RPs. In all eukaryote groups tested, the LSU RPs have significantly higher content of basic residues and homobasic segments than the SSU RPs. The vertebrate LSU RPs have much higher sequestration of basic residues than those of bacteria, archaea and even of the lower eukarya. The basic clusters are highly aligned in the vertebrate, but less in the lower eukarya, and only within families in archaea and bacteria. Increase in the basicity of RPs, besides helping transport to the nucleus, should promote stability of the assembled ribosome as well as the association with translocons and other intracellular matrix proteins. The size and GC nucleotide bias of the expansion segments of large LSU rRNAs also culminate in the vertebrate, and should support ribosome association with the endoplasmic reticulum and other intracellular networks. However, the expansion and nucleotide bias of eukaryote LSU rRNAs do not clearly correlate with changes in ionic parameters of LSU ribosomal proteins.

Original languageEnglish (US)
Pages (from-to)1589-1604
Number of pages16
JournalAmino Acids
Volume46
Issue number7
DOIs
StatePublished - Jan 1 2014

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Ribosomal RNA
Ribosomal Proteins
Eukaryota
Archaea
Ribosomes
Vertebrates
Bacteria
Eukaryotic Large Ribosome Subunits
Nucleotides
Large Ribosome Subunits
Association reactions
Biota
Constitution and Bylaws
Hydrophobicity
Alkalinity
Hydrophobic and Hydrophilic Interactions
Endoplasmic Reticulum
Cluster Analysis

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Clinical Biochemistry
  • Organic Chemistry

Cite this

Parker, M. S., Sah, R., Balasubramaniam, A., Sallee, F. R., Park, E., & Parker, S. L. (2014). On the expansion of ribosomal proteins and RNAs in eukaryotes. Amino Acids, 46(7), 1589-1604. https://doi.org/10.1007/s00726-014-1704-4

On the expansion of ribosomal proteins and RNAs in eukaryotes. / Parker, Michael S.; Sah, Renu; Balasubramaniam, Ambikaipakan; Sallee, Floyd R.; Park, Edwards; Parker, Steven L.

In: Amino Acids, Vol. 46, No. 7, 01.01.2014, p. 1589-1604.

Research output: Contribution to journalReview article

Parker, MS, Sah, R, Balasubramaniam, A, Sallee, FR, Park, E & Parker, SL 2014, 'On the expansion of ribosomal proteins and RNAs in eukaryotes', Amino Acids, vol. 46, no. 7, pp. 1589-1604. https://doi.org/10.1007/s00726-014-1704-4
Parker MS, Sah R, Balasubramaniam A, Sallee FR, Park E, Parker SL. On the expansion of ribosomal proteins and RNAs in eukaryotes. Amino Acids. 2014 Jan 1;46(7):1589-1604. https://doi.org/10.1007/s00726-014-1704-4
Parker, Michael S. ; Sah, Renu ; Balasubramaniam, Ambikaipakan ; Sallee, Floyd R. ; Park, Edwards ; Parker, Steven L. / On the expansion of ribosomal proteins and RNAs in eukaryotes. In: Amino Acids. 2014 ; Vol. 46, No. 7. pp. 1589-1604.
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