On the structural basis of modal gating behavior in K+ channels

Sudha Chakrapani, Julio Cordero-Morales, Vishwanath Jogini, Albert C. Pan, D. Marien Cortes, Benoît Roux, Eduardo Perozo

Research output: Contribution to journalArticle

43 Citations (Scopus)

Abstract

Modal-gating shifts represent an effective regulatory mechanism by which ion channels control the extent and time course of ionic fluxes. Under steady-state conditions, the K+ channel KcsA shows three distinct gating modes, high-Po, low-Po and a high-frequency flicker mode, each with about an order of magnitude difference in their mean open times. Here we show that in the absence of C-type inactivation, mutations at the pore-helix position Glu71 unmask a series of kinetically distinct modes of gating in a side chain-specific way. These gating modes mirror those seen in wild-type channels and suggest that specific interactions in the side chain network surrounding the selectivity filter, in concert with ion occupancy, alter the relative stability of pre-existing conformational states of the pore. The present results highlight the key role of the selectivity filter in regulating modal gating behavior in K + channels.

Original languageEnglish (US)
Pages (from-to)67-75
Number of pages9
JournalNature Structural and Molecular Biology
Volume18
Issue number1
DOIs
StatePublished - Jan 1 2011

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Ion Channels
Ions
Mutation

All Science Journal Classification (ASJC) codes

  • Structural Biology
  • Molecular Biology

Cite this

Chakrapani, S., Cordero-Morales, J., Jogini, V., Pan, A. C., Cortes, D. M., Roux, B., & Perozo, E. (2011). On the structural basis of modal gating behavior in K+ channels. Nature Structural and Molecular Biology, 18(1), 67-75. https://doi.org/10.1038/nsmb.1968

On the structural basis of modal gating behavior in K+ channels. / Chakrapani, Sudha; Cordero-Morales, Julio; Jogini, Vishwanath; Pan, Albert C.; Cortes, D. Marien; Roux, Benoît; Perozo, Eduardo.

In: Nature Structural and Molecular Biology, Vol. 18, No. 1, 01.01.2011, p. 67-75.

Research output: Contribution to journalArticle

Chakrapani, S, Cordero-Morales, J, Jogini, V, Pan, AC, Cortes, DM, Roux, B & Perozo, E 2011, 'On the structural basis of modal gating behavior in K+ channels', Nature Structural and Molecular Biology, vol. 18, no. 1, pp. 67-75. https://doi.org/10.1038/nsmb.1968
Chakrapani, Sudha ; Cordero-Morales, Julio ; Jogini, Vishwanath ; Pan, Albert C. ; Cortes, D. Marien ; Roux, Benoît ; Perozo, Eduardo. / On the structural basis of modal gating behavior in K+ channels. In: Nature Structural and Molecular Biology. 2011 ; Vol. 18, No. 1. pp. 67-75.
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