Opioid and tachykinin neuropeptides in prolactin-secreting human pituitary adenomas

Xuegong Zhu, James T. Robertson, Harold S. Sacks, F Dohan, Jih Lie Tseng, Dominic M. Desiderio

Research output: Contribution to journalArticle

25 Citations (Scopus)

Abstract

Two opioid neuropeptides, methionine enkephalin (ME) and β-endorphin (BE), and one tachykinin neuropeptide, substance P (SP), were quantified in 10 prolactin (PRL)-secreting human pituitary adenomas and in 10 control human pituitaries. Immunohistochemical techniques provided appropriate staining for PRL. Reversed-phase high performance liquid chromatography (RP-HPLC) was used to purify these three neuropeptides before their analysis, radioimmunoassay (RIA) was used for the quantification of SP-like immunoreactivity (SP-LI), and liquid secondary-ion mass spectrometry (LSIMS) was used for the qualitative and quantitative analysis of ME and a tryptic peptide of BE. This study shows that, for 90% of the cases studied here (excluding one hypothyroidism case), the tachykinin A neuropeptide SP-LI level is decreased, the POMC peptide BE level is not altered, and the proenkephalin A neuropeptide ME level is increased in these PRL-secreting tumors.

Original languageEnglish (US)
Pages (from-to)1097-1107
Number of pages11
JournalPeptides
Volume16
Issue number6
DOIs
StatePublished - Jan 1 1995

Fingerprint

Prolactinoma
Tachykinins
Neuropeptides
Prolactin
Opioid Analgesics
Substance P
Methionine Enkephalin
Secondary Ion Mass Spectrometry
Endorphins
Pro-Opiomelanocortin
Peptides
High performance liquid chromatography
Reverse-Phase Chromatography
Secondary ion mass spectrometry
Hypothyroidism
Radioimmunoassay
Tumors
High Pressure Liquid Chromatography
Staining and Labeling
Liquids

All Science Journal Classification (ASJC) codes

  • Physiology
  • Biochemistry
  • Endocrinology
  • Cellular and Molecular Neuroscience

Cite this

Zhu, X., Robertson, J. T., Sacks, H. S., Dohan, F., Tseng, J. L., & Desiderio, D. M. (1995). Opioid and tachykinin neuropeptides in prolactin-secreting human pituitary adenomas. Peptides, 16(6), 1097-1107. https://doi.org/10.1016/0196-9781(95)00081-T

Opioid and tachykinin neuropeptides in prolactin-secreting human pituitary adenomas. / Zhu, Xuegong; Robertson, James T.; Sacks, Harold S.; Dohan, F; Tseng, Jih Lie; Desiderio, Dominic M.

In: Peptides, Vol. 16, No. 6, 01.01.1995, p. 1097-1107.

Research output: Contribution to journalArticle

Zhu, X, Robertson, JT, Sacks, HS, Dohan, F, Tseng, JL & Desiderio, DM 1995, 'Opioid and tachykinin neuropeptides in prolactin-secreting human pituitary adenomas', Peptides, vol. 16, no. 6, pp. 1097-1107. https://doi.org/10.1016/0196-9781(95)00081-T
Zhu, Xuegong ; Robertson, James T. ; Sacks, Harold S. ; Dohan, F ; Tseng, Jih Lie ; Desiderio, Dominic M. / Opioid and tachykinin neuropeptides in prolactin-secreting human pituitary adenomas. In: Peptides. 1995 ; Vol. 16, No. 6. pp. 1097-1107.
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