Opioids activate G proteins in REM sleep-related brain stem nuclei of rat

M. Luisa Capece, Helen Baghdoyan, Ralph Lydic

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

Mu opioid receptors within the pontine reticular formation contribute to opioid-induced rapid eye movement (REM) sleep inhibition. Mu receptors are coupled to guanine nucleotide binding (G) proteins and this study tested the hypothesis that the μ opioid agonist [D-Ala2,N-Me-Phe4,Gly-ol5]enkephalin (DAMGO) would activate G proteins in rat brain stem nuclei known to regulate REM sleep. In vitro autoradiography of DAMGO-stimulated [35S]GTPγS binding showed that, compared with basal [35S]GTPγS binding, DAMGO significantly increased G protein activation in the nucleus pontis oralis (56.2%), nucleus pontis caudalis (57.3%), laterodorsal tegmental nucleus (75.8%), pedunculopontine tegmental nucleus (72.4%), nucleus locus coeruleus (77.2%) and dorsal raphe nucleus (73.4%). DAMGO stimulation of [35S]GTPγS binding in nuclei regulating REM sleep suggests that opioid-induced REM sleep inhibition involves activation of G proteins.

Original languageEnglish (US)
Pages (from-to)3025-3028
Number of pages4
JournalNeuroReport
Volume9
Issue number13
DOIs
StatePublished - Sep 14 1998
Externally publishedYes

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Enkephalins
REM Sleep
GTP-Binding Proteins
Opioid Analgesics
Brain Stem
Sleep
mu Opioid Receptor
Pedunculopontine Tegmental Nucleus
Guanine Nucleotides
Locus Coeruleus
Autoradiography
Carrier Proteins
Inhibition (Psychology)

All Science Journal Classification (ASJC) codes

  • Neuroscience(all)

Cite this

Opioids activate G proteins in REM sleep-related brain stem nuclei of rat. / Capece, M. Luisa; Baghdoyan, Helen; Lydic, Ralph.

In: NeuroReport, Vol. 9, No. 13, 14.09.1998, p. 3025-3028.

Research output: Contribution to journalArticle

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