Opioids in cerebrospinal fluid in hypotensive newborn pigs

W. M. Armstead, R. Mirro, D. W. Busija, D. M. Desiderio, Charles Leffler

Research output: Contribution to journalArticle

42 Citations (Scopus)

Abstract

This study was designed to determine if opioids were detectable in cerebrospinal fluid (CSF) and if these concentrations were altered by hemorrhagic hypotension. This study was further designed to determine the effects of topically administered opioids on pial arteriolar diameter during normotension and hypotension. Closed cranial windows were used to determine pial arteriolar diameter. Periarachnoid cortical and cisterna magna CSF was collected from piglets during normotension and hypotension (systemic arterial pressure decreased from 63 ± 1 to 33 ± 1 mm Hg). Opioid profiles were assessed qualitatively by radioreceptor assay, and individual opioids were measured quantitatively by radioimmunoassay. Periarachnoid cortical and cisterna magna CSF methionine enkephalin-, leucine enkephalin-, dynorphin-, and β-endorphin-like receptor active values all were increased by hypotension. When quantified by radioimmunoassay, periarachnoid cortical CSF values for methionine enkephalin-like immunoreactivity were 1,167 ± 58 and 2,975 ± 139 pg/ml for normotension and hypotension, respectively. Periarachnoid cortical CSF radioimmunoassay values for dynorphin-like immunoreactivity were 15 ± 2 and 28 ± 2 pg/ml for normotension and hypotension, respectively. When applied topically to the cortical surface, synthetic methionine enkephalin increased pial arteriolar diameter (134 ± 4, 158 ± 4, and 163 ± 4 μm for control, 574 pg/ml [10-10 M], and 5,740 pg/ml [10-9 M], respectively). Similarly, topical synthetic leucine enkephalin and dynorphin elicited pial arteriolar dilation. However, β-endorphin produced arteriolar constriction. Hypotension attenuated methionine aud leucine enkephalin-induced dilation and reversed dynorphin-induced dilation to concentration-dependent constriction. β-Endorphin-induced constriction was not changed by hypotension. Therefore, opioids could contribute to the control of the cerebral circulation during hypotension.

Original languageEnglish (US)
Pages (from-to)922-929
Number of pages8
JournalCirculation research
Volume68
Issue number4
DOIs
StatePublished - Jan 1 1991

Fingerprint

Hypotension
Opioid Analgesics
Cerebrospinal Fluid
Swine
Dynorphins
Leucine Enkephalin
Methionine Enkephalin
Constriction
Cisterna Magna
Endorphins
Radioimmunoassay
Dilatation
Cerebrovascular Circulation
Radioligand Assay
Opioid Receptors
Methionine
Arterial Pressure

All Science Journal Classification (ASJC) codes

  • Physiology
  • Cardiology and Cardiovascular Medicine

Cite this

Armstead, W. M., Mirro, R., Busija, D. W., Desiderio, D. M., & Leffler, C. (1991). Opioids in cerebrospinal fluid in hypotensive newborn pigs. Circulation research, 68(4), 922-929. https://doi.org/10.1161/01.RES.68.4.922

Opioids in cerebrospinal fluid in hypotensive newborn pigs. / Armstead, W. M.; Mirro, R.; Busija, D. W.; Desiderio, D. M.; Leffler, Charles.

In: Circulation research, Vol. 68, No. 4, 01.01.1991, p. 922-929.

Research output: Contribution to journalArticle

Armstead, WM, Mirro, R, Busija, DW, Desiderio, DM & Leffler, C 1991, 'Opioids in cerebrospinal fluid in hypotensive newborn pigs', Circulation research, vol. 68, no. 4, pp. 922-929. https://doi.org/10.1161/01.RES.68.4.922
Armstead, W. M. ; Mirro, R. ; Busija, D. W. ; Desiderio, D. M. ; Leffler, Charles. / Opioids in cerebrospinal fluid in hypotensive newborn pigs. In: Circulation research. 1991 ; Vol. 68, No. 4. pp. 922-929.
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