Opposing functions of calcineurin and CaMKII regulate G-protein signaling in egg-laying behavior of C. elegans

Jungsoo Lee, Chang Hoon Jee, Hyun Ok Song, Jaya Bandyopadhyay, Jin Il Lee, Jae Ran Yu, Jiyeon Lee, Byung Jae Park, Joohong Ahnn

Research output: Contribution to journalArticle

25 Citations (Scopus)

Abstract

Ca 2+/calmodulin-dependent calcineurin has been shown to have important roles in various Ca 2+ signaling pathways. We have previously reported that cnb-1(jh103) mutants, null mutants of a regulatory B subunit, displayed pleiotropic defects including uncoordinated movement and delayed egg laying in Caenorhabditis elegans. Interestingly, gain-of-function mutants of a catalytic A subunit showed exactly opposite phenotypes to those of cnb-1(null) mutants providing an excellent genetic model to define calcium-mediated signaling pathway at the organism level. Furthermore, calcineurin is also important for normal cuticle formation, which is required for maintenance of normal body size in C. elegans. Genetic interactions between tax-6 and several mutants including egl-30 and egl-10, which are known to be involved in G-protein signaling pathways suggest that calcineurin indeed regulates locomotion and serotonin-mediated egg laying through goa-1(Goα) and egl-30(Gqα). Our results indicate that, along with CaMKII, calcineurin regulates G-protein-coupled phosphorylation signaling pathways in C. elegans.

Original languageEnglish (US)
Pages (from-to)585-595
Number of pages11
JournalJournal of Molecular Biology
Volume344
Issue number2
DOIs
StatePublished - Nov 19 2004
Externally publishedYes

Fingerprint

Calcium-Calmodulin-Dependent Protein Kinase Type 2
Calcineurin
GTP-Binding Proteins
Ovum
Caenorhabditis elegans
Calcium Signaling
Genetic Models
Body Size
Calmodulin
Locomotion
Catalytic Domain
Serotonin
Maintenance
Phosphorylation
Phenotype

All Science Journal Classification (ASJC) codes

  • Structural Biology
  • Molecular Biology

Cite this

Opposing functions of calcineurin and CaMKII regulate G-protein signaling in egg-laying behavior of C. elegans. / Lee, Jungsoo; Jee, Chang Hoon; Song, Hyun Ok; Bandyopadhyay, Jaya; Lee, Jin Il; Yu, Jae Ran; Lee, Jiyeon; Park, Byung Jae; Ahnn, Joohong.

In: Journal of Molecular Biology, Vol. 344, No. 2, 19.11.2004, p. 585-595.

Research output: Contribution to journalArticle

Lee, Jungsoo ; Jee, Chang Hoon ; Song, Hyun Ok ; Bandyopadhyay, Jaya ; Lee, Jin Il ; Yu, Jae Ran ; Lee, Jiyeon ; Park, Byung Jae ; Ahnn, Joohong. / Opposing functions of calcineurin and CaMKII regulate G-protein signaling in egg-laying behavior of C. elegans. In: Journal of Molecular Biology. 2004 ; Vol. 344, No. 2. pp. 585-595.
@article{5a89b5834dec477491297c2eeb876972,
title = "Opposing functions of calcineurin and CaMKII regulate G-protein signaling in egg-laying behavior of C. elegans",
abstract = "Ca 2+/calmodulin-dependent calcineurin has been shown to have important roles in various Ca 2+ signaling pathways. We have previously reported that cnb-1(jh103) mutants, null mutants of a regulatory B subunit, displayed pleiotropic defects including uncoordinated movement and delayed egg laying in Caenorhabditis elegans. Interestingly, gain-of-function mutants of a catalytic A subunit showed exactly opposite phenotypes to those of cnb-1(null) mutants providing an excellent genetic model to define calcium-mediated signaling pathway at the organism level. Furthermore, calcineurin is also important for normal cuticle formation, which is required for maintenance of normal body size in C. elegans. Genetic interactions between tax-6 and several mutants including egl-30 and egl-10, which are known to be involved in G-protein signaling pathways suggest that calcineurin indeed regulates locomotion and serotonin-mediated egg laying through goa-1(Goα) and egl-30(Gqα). Our results indicate that, along with CaMKII, calcineurin regulates G-protein-coupled phosphorylation signaling pathways in C. elegans.",
author = "Jungsoo Lee and Jee, {Chang Hoon} and Song, {Hyun Ok} and Jaya Bandyopadhyay and Lee, {Jin Il} and Yu, {Jae Ran} and Jiyeon Lee and Park, {Byung Jae} and Joohong Ahnn",
year = "2004",
month = "11",
day = "19",
doi = "10.1016/j.jmb.2004.09.050",
language = "English (US)",
volume = "344",
pages = "585--595",
journal = "Journal of Molecular Biology",
issn = "0022-2836",
publisher = "Academic Press Inc.",
number = "2",

}

TY - JOUR

T1 - Opposing functions of calcineurin and CaMKII regulate G-protein signaling in egg-laying behavior of C. elegans

AU - Lee, Jungsoo

AU - Jee, Chang Hoon

AU - Song, Hyun Ok

AU - Bandyopadhyay, Jaya

AU - Lee, Jin Il

AU - Yu, Jae Ran

AU - Lee, Jiyeon

AU - Park, Byung Jae

AU - Ahnn, Joohong

PY - 2004/11/19

Y1 - 2004/11/19

N2 - Ca 2+/calmodulin-dependent calcineurin has been shown to have important roles in various Ca 2+ signaling pathways. We have previously reported that cnb-1(jh103) mutants, null mutants of a regulatory B subunit, displayed pleiotropic defects including uncoordinated movement and delayed egg laying in Caenorhabditis elegans. Interestingly, gain-of-function mutants of a catalytic A subunit showed exactly opposite phenotypes to those of cnb-1(null) mutants providing an excellent genetic model to define calcium-mediated signaling pathway at the organism level. Furthermore, calcineurin is also important for normal cuticle formation, which is required for maintenance of normal body size in C. elegans. Genetic interactions between tax-6 and several mutants including egl-30 and egl-10, which are known to be involved in G-protein signaling pathways suggest that calcineurin indeed regulates locomotion and serotonin-mediated egg laying through goa-1(Goα) and egl-30(Gqα). Our results indicate that, along with CaMKII, calcineurin regulates G-protein-coupled phosphorylation signaling pathways in C. elegans.

AB - Ca 2+/calmodulin-dependent calcineurin has been shown to have important roles in various Ca 2+ signaling pathways. We have previously reported that cnb-1(jh103) mutants, null mutants of a regulatory B subunit, displayed pleiotropic defects including uncoordinated movement and delayed egg laying in Caenorhabditis elegans. Interestingly, gain-of-function mutants of a catalytic A subunit showed exactly opposite phenotypes to those of cnb-1(null) mutants providing an excellent genetic model to define calcium-mediated signaling pathway at the organism level. Furthermore, calcineurin is also important for normal cuticle formation, which is required for maintenance of normal body size in C. elegans. Genetic interactions between tax-6 and several mutants including egl-30 and egl-10, which are known to be involved in G-protein signaling pathways suggest that calcineurin indeed regulates locomotion and serotonin-mediated egg laying through goa-1(Goα) and egl-30(Gqα). Our results indicate that, along with CaMKII, calcineurin regulates G-protein-coupled phosphorylation signaling pathways in C. elegans.

UR - http://www.scopus.com/inward/record.url?scp=7444226841&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=7444226841&partnerID=8YFLogxK

U2 - 10.1016/j.jmb.2004.09.050

DO - 10.1016/j.jmb.2004.09.050

M3 - Article

VL - 344

SP - 585

EP - 595

JO - Journal of Molecular Biology

JF - Journal of Molecular Biology

SN - 0022-2836

IS - 2

ER -