Optical study of stress hormone-induced nanoscale structural alteration in brain using partial wave spectroscopic microscopy

Shiva Bhandari, Pradeep Kumar Shukla, Huda M. Almabadi, Peeyush Sahay, Radhakrishna Rao, Prabhakar Pradhan

Research output: Contribution to journalArticle

Abstract

Chronic stress affects nano to microscale structures of the brain cells/tissues due to suppression of neural growths and reconnections, hence the neuronal activities. This results in depression, memory loss and even death of the brain cells. Our recently developed novel optical technique, partial wave spectroscopic microscopy has nanoscale sensitivity, and hence, can detect nanoscale changes in brain tissues due to stress. In this study, we applied this technique to quantify the stress related structural changes in the corticosterone-treated mouse model of stress. Our results show that brains from corticosterone-treated mice showed higher nanoscale structural disorder in the hippocampal region as compared to the brain from normal (vehicle) mice. The increase in structural alteration correlates with the duration of the stress. We further quantified the relative changes and the spatial localization of these changes in this mouse model and found out that the maximum changes occurred nearly symmetrically in both regions of the hippocampus. The mRNA for stress-related genes, brain-derived neurotrophic factor and tyrosine kinase-coupled receptor were also significantly reduced in the hippocampus of corticosterone-treated mice compared to that in control mice. These results indicate that chronic corticosterone treatment induces nanoscale structural alterations in mouse brain that corresponds to changes in stress-related gene expression.

Original languageEnglish (US)
Article numbere201800002
JournalJournal of Biophotonics
Volume12
Issue number6
DOIs
StatePublished - Jun 1 2019

Fingerprint

hormones
Hormones
brain
mice
Microscopy
Brain
Microscopic examination
Corticosterone
microscopy
hippocampus
Hippocampus
Tissue
Brain-Derived Neurotrophic Factor
Brain Death
gene expression
tyrosine
Gene expression
Memory Disorders
Protein-Tyrosine Kinases
Receptor Protein-Tyrosine Kinases

All Science Journal Classification (ASJC) codes

  • Chemistry(all)
  • Materials Science(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Engineering(all)
  • Physics and Astronomy(all)

Cite this

Optical study of stress hormone-induced nanoscale structural alteration in brain using partial wave spectroscopic microscopy. / Bhandari, Shiva; Shukla, Pradeep Kumar; Almabadi, Huda M.; Sahay, Peeyush; Rao, Radhakrishna; Pradhan, Prabhakar.

In: Journal of Biophotonics, Vol. 12, No. 6, e201800002, 01.06.2019.

Research output: Contribution to journalArticle

Bhandari, Shiva ; Shukla, Pradeep Kumar ; Almabadi, Huda M. ; Sahay, Peeyush ; Rao, Radhakrishna ; Pradhan, Prabhakar. / Optical study of stress hormone-induced nanoscale structural alteration in brain using partial wave spectroscopic microscopy. In: Journal of Biophotonics. 2019 ; Vol. 12, No. 6.
@article{7fd3da94fb9a410eaff5d3361271d533,
title = "Optical study of stress hormone-induced nanoscale structural alteration in brain using partial wave spectroscopic microscopy",
abstract = "Chronic stress affects nano to microscale structures of the brain cells/tissues due to suppression of neural growths and reconnections, hence the neuronal activities. This results in depression, memory loss and even death of the brain cells. Our recently developed novel optical technique, partial wave spectroscopic microscopy has nanoscale sensitivity, and hence, can detect nanoscale changes in brain tissues due to stress. In this study, we applied this technique to quantify the stress related structural changes in the corticosterone-treated mouse model of stress. Our results show that brains from corticosterone-treated mice showed higher nanoscale structural disorder in the hippocampal region as compared to the brain from normal (vehicle) mice. The increase in structural alteration correlates with the duration of the stress. We further quantified the relative changes and the spatial localization of these changes in this mouse model and found out that the maximum changes occurred nearly symmetrically in both regions of the hippocampus. The mRNA for stress-related genes, brain-derived neurotrophic factor and tyrosine kinase-coupled receptor were also significantly reduced in the hippocampus of corticosterone-treated mice compared to that in control mice. These results indicate that chronic corticosterone treatment induces nanoscale structural alterations in mouse brain that corresponds to changes in stress-related gene expression.",
author = "Shiva Bhandari and Shukla, {Pradeep Kumar} and Almabadi, {Huda M.} and Peeyush Sahay and Radhakrishna Rao and Prabhakar Pradhan",
year = "2019",
month = "6",
day = "1",
doi = "10.1002/jbio.201800002",
language = "English (US)",
volume = "12",
journal = "Journal of Biophotonics",
issn = "1864-063X",
publisher = "Wiley-VCH Verlag",
number = "6",

}

TY - JOUR

T1 - Optical study of stress hormone-induced nanoscale structural alteration in brain using partial wave spectroscopic microscopy

AU - Bhandari, Shiva

AU - Shukla, Pradeep Kumar

AU - Almabadi, Huda M.

AU - Sahay, Peeyush

AU - Rao, Radhakrishna

AU - Pradhan, Prabhakar

PY - 2019/6/1

Y1 - 2019/6/1

N2 - Chronic stress affects nano to microscale structures of the brain cells/tissues due to suppression of neural growths and reconnections, hence the neuronal activities. This results in depression, memory loss and even death of the brain cells. Our recently developed novel optical technique, partial wave spectroscopic microscopy has nanoscale sensitivity, and hence, can detect nanoscale changes in brain tissues due to stress. In this study, we applied this technique to quantify the stress related structural changes in the corticosterone-treated mouse model of stress. Our results show that brains from corticosterone-treated mice showed higher nanoscale structural disorder in the hippocampal region as compared to the brain from normal (vehicle) mice. The increase in structural alteration correlates with the duration of the stress. We further quantified the relative changes and the spatial localization of these changes in this mouse model and found out that the maximum changes occurred nearly symmetrically in both regions of the hippocampus. The mRNA for stress-related genes, brain-derived neurotrophic factor and tyrosine kinase-coupled receptor were also significantly reduced in the hippocampus of corticosterone-treated mice compared to that in control mice. These results indicate that chronic corticosterone treatment induces nanoscale structural alterations in mouse brain that corresponds to changes in stress-related gene expression.

AB - Chronic stress affects nano to microscale structures of the brain cells/tissues due to suppression of neural growths and reconnections, hence the neuronal activities. This results in depression, memory loss and even death of the brain cells. Our recently developed novel optical technique, partial wave spectroscopic microscopy has nanoscale sensitivity, and hence, can detect nanoscale changes in brain tissues due to stress. In this study, we applied this technique to quantify the stress related structural changes in the corticosterone-treated mouse model of stress. Our results show that brains from corticosterone-treated mice showed higher nanoscale structural disorder in the hippocampal region as compared to the brain from normal (vehicle) mice. The increase in structural alteration correlates with the duration of the stress. We further quantified the relative changes and the spatial localization of these changes in this mouse model and found out that the maximum changes occurred nearly symmetrically in both regions of the hippocampus. The mRNA for stress-related genes, brain-derived neurotrophic factor and tyrosine kinase-coupled receptor were also significantly reduced in the hippocampus of corticosterone-treated mice compared to that in control mice. These results indicate that chronic corticosterone treatment induces nanoscale structural alterations in mouse brain that corresponds to changes in stress-related gene expression.

UR - http://www.scopus.com/inward/record.url?scp=85063281300&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85063281300&partnerID=8YFLogxK

U2 - 10.1002/jbio.201800002

DO - 10.1002/jbio.201800002

M3 - Article

VL - 12

JO - Journal of Biophotonics

JF - Journal of Biophotonics

SN - 1864-063X

IS - 6

M1 - e201800002

ER -