Optimization of cAMP fluorescence dataset from ACTOne cannabinoid receptor 1 cell line

Chaela S. Presley, Ammaar Abidi, Bob Moore

Research output: Contribution to journalArticle

Abstract

The ACTOne cannabinoid receptor 1 functional system is comprised of transfected HEK cells with the parental cyclic nucleotide gated channel (CNG) co-transfected with cannabinoid receptor 1 (CB1). The ACTOne CB1 cell line was evaluated for cAMP driven fluorescence by optimizing experimental conditions for sensitivity to forskolin and CP 55,940, reading time point, reliability of cell passage number, and pertussis inactivation of Gi/o.

Original languageEnglish (US)
Pages (from-to)1118-1123
Number of pages6
JournalData in Brief
Volume7
DOIs
StatePublished - Jun 1 2016

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Optimization of cAMP fluorescence dataset from ACTOne cannabinoid receptor 1 cell line. / Presley, Chaela S.; Abidi, Ammaar; Moore, Bob.

In: Data in Brief, Vol. 7, 01.06.2016, p. 1118-1123.

Research output: Contribution to journalArticle

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