Optimizations of Radiolabeling of Immunoproteins with 213Bi

Rose A. Boll, Saed Mirzadeh, Stephen Kennel

Research output: Contribution to journalArticle

36 Citations (Scopus)

Abstract

Carrier-free 213Bi ((t1/3 = 45.6 m, an α-emitter) is available from the 225Ra/225 Ac/213Bi generator system with 225Ra (t1/2 = 14 d) and 225Ac (t1/3 = 10 d) being the daughters of 7340-y 229Th which, in turn, is the decay daughter of long-lived 233U. In the current generator system, 225Ra/225Ac is adsorbed on an organic cation exchange resin (AG 50X4, X8 or highly cross-linked AG MP-50) and 213Bi is eluted with 0.1 M HI. We have found the lower cross-linked resin to be more suitable for rapid elution of 213Bi from the generator with 225Ac breakthrough of <1%. Generators were typically made from Ac separated from Ra as the breakthrough of Ra is significantly higher than that of Ac. Generator elution of 213Bi, labeling of MAb and HPLC purification were accomplished within 35-40 min. Radiolabeling yields of CHX-b-DTPA-conjugated MAbs (34A and 14) were >50% with specific activity of ∼200 μCi of 213Bi per 150 μg of conjugated protein.

Original languageEnglish (US)
Pages (from-to)145-149
Number of pages5
JournalRadiochimica Acta
Volume79
Issue number2
DOIs
StatePublished - Jan 1 1997
Externally publishedYes

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Immunoproteins
generators
Cation Exchange Resins
resins
optimization
elution
Resins
emitters
proteins
cations
Proteins
decay
3-monoiodothyronine

All Science Journal Classification (ASJC) codes

  • Physical and Theoretical Chemistry

Cite this

Optimizations of Radiolabeling of Immunoproteins with 213Bi. / Boll, Rose A.; Mirzadeh, Saed; Kennel, Stephen.

In: Radiochimica Acta, Vol. 79, No. 2, 01.01.1997, p. 145-149.

Research output: Contribution to journalArticle

Boll, Rose A. ; Mirzadeh, Saed ; Kennel, Stephen. / Optimizations of Radiolabeling of Immunoproteins with 213Bi. In: Radiochimica Acta. 1997 ; Vol. 79, No. 2. pp. 145-149.
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