ORP150 protects against hypoxia/ischemia-induced neuronal death

Michio Tamatani, Tomohiro Matsuyama, Atsushi Yamaguchi, Noriaki Mitsuda, Yoshitane Tsukamoto, Manabu Taniguchi, Yong Ho Che, Kentaro Ozawa, Osamu Hori, Hiroyuki Nishimura, Atsuko Yamashita, Masaru Okabe, Hideki Yanagi, David Stern, Satoshi Ogawa, Masaya Tohyama

Research output: Contribution to journalArticle

159 Citations (Scopus)

Abstract

Oxygen-regulated protein 150 kD (ORP150) is a novel endoplasmic-reticulum-associated chaperone induced by hypoxia/ischemia. Although ORP150 was sparingly upregulated in neurons from human brain undergoing ischemic stress, there was robust induction in astrocytes. Cultured neurons overexpressing ORP150 were resistant to hypoxemic stress, whereas astrocytes with inhibited ORP150 expression were more vulnerable. Mice with targeted neuronal overexpression of ORP150 had smaller strokes compared with controls. Neurons with increased ORP150 demonstrated suppressed caspase-3-like activity and enhanced brain-derived neurotrophic factor (BDNF) under hypoxia signaling. These data indicate that ORP150 is an integral participant in ischemic cytoprotective pathways.

Original languageEnglish (US)
Pages (from-to)317-323
Number of pages7
JournalNature Medicine
Volume7
Issue number3
DOIs
StatePublished - Mar 29 2001
Externally publishedYes

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Ischemia
Neurons
Astrocytes
Brain-Derived Neurotrophic Factor
oxygen-regulated proteins
Hypoxia
Caspase 3
Endoplasmic Reticulum
Brain
Stroke

All Science Journal Classification (ASJC) codes

  • Biochemistry, Genetics and Molecular Biology(all)

Cite this

Tamatani, M., Matsuyama, T., Yamaguchi, A., Mitsuda, N., Tsukamoto, Y., Taniguchi, M., ... Tohyama, M. (2001). ORP150 protects against hypoxia/ischemia-induced neuronal death. Nature Medicine, 7(3), 317-323. https://doi.org/10.1038/85463

ORP150 protects against hypoxia/ischemia-induced neuronal death. / Tamatani, Michio; Matsuyama, Tomohiro; Yamaguchi, Atsushi; Mitsuda, Noriaki; Tsukamoto, Yoshitane; Taniguchi, Manabu; Che, Yong Ho; Ozawa, Kentaro; Hori, Osamu; Nishimura, Hiroyuki; Yamashita, Atsuko; Okabe, Masaru; Yanagi, Hideki; Stern, David; Ogawa, Satoshi; Tohyama, Masaya.

In: Nature Medicine, Vol. 7, No. 3, 29.03.2001, p. 317-323.

Research output: Contribution to journalArticle

Tamatani, M, Matsuyama, T, Yamaguchi, A, Mitsuda, N, Tsukamoto, Y, Taniguchi, M, Che, YH, Ozawa, K, Hori, O, Nishimura, H, Yamashita, A, Okabe, M, Yanagi, H, Stern, D, Ogawa, S & Tohyama, M 2001, 'ORP150 protects against hypoxia/ischemia-induced neuronal death', Nature Medicine, vol. 7, no. 3, pp. 317-323. https://doi.org/10.1038/85463
Tamatani M, Matsuyama T, Yamaguchi A, Mitsuda N, Tsukamoto Y, Taniguchi M et al. ORP150 protects against hypoxia/ischemia-induced neuronal death. Nature Medicine. 2001 Mar 29;7(3):317-323. https://doi.org/10.1038/85463
Tamatani, Michio ; Matsuyama, Tomohiro ; Yamaguchi, Atsushi ; Mitsuda, Noriaki ; Tsukamoto, Yoshitane ; Taniguchi, Manabu ; Che, Yong Ho ; Ozawa, Kentaro ; Hori, Osamu ; Nishimura, Hiroyuki ; Yamashita, Atsuko ; Okabe, Masaru ; Yanagi, Hideki ; Stern, David ; Ogawa, Satoshi ; Tohyama, Masaya. / ORP150 protects against hypoxia/ischemia-induced neuronal death. In: Nature Medicine. 2001 ; Vol. 7, No. 3. pp. 317-323.
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