Osteoarthritic lesions

Involvement of three different collagenases

Boris V. Shlopov, Wen Rong Lie, Carlo L. Mainardi, Ada A. Cole, Susan Chubinskaya, Karen Hasty

Research output: Contribution to journalArticle

247 Citations (Scopus)

Abstract

Objective. To assess the presence of fibroblast collagenase (MMP-1), neutrophil collagenase (MMP-8), and collagenase 3 (MMP-13) in osteoarthritic (OA) cartilage, with particular emphasis on areas of macroscopic cartilage erosion. Methods. Messenger RNA (mRNA) levels were assessed by reverse transcriptase-polymerase chain reaction (RT-PCR), in situ hybridization, and Northern blot analysis. Results. MMP-1 and MMP-13 were expressed at higher levels by OA chondrocytes than by normal chondrocytes. In addition, mRNA for MMP-8 was present in OA cartilage but not normal cartilage by PCR and Northern blot analyses. Chondrocytes from areas surrounding the OA lesion expressed greater quantities of MMP-1 and MMP-13 compared with normal chondrocytes, suggesting local modulation by mechanical and inflammatory factors. Tumor necrosis factor α stimulated the expression of all 3 collagenases. Retinoic acid, an agent which induces autodigestion of cartilage in vitro, stimulated only the expression of MMP-13. Conclusion. These findings suggest a key role of MMP-13 and MMP-8, as well as MMP-1 in osteoarthritis.

Original languageEnglish (US)
Pages (from-to)2065-2074
Number of pages10
JournalArthritis and rheumatism
Volume40
Issue number11
DOIs
StatePublished - Nov 1 1997

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Collagenases
Matrix Metalloproteinases
Cartilage
Chondrocytes
Matrix Metalloproteinase 8
Matrix Metalloproteinase 13
Northern Blotting
Messenger RNA
Tretinoin
Reverse Transcriptase Polymerase Chain Reaction
Osteoarthritis
In Situ Hybridization
Tumor Necrosis Factor-alpha

All Science Journal Classification (ASJC) codes

  • Immunology and Allergy
  • Rheumatology
  • Immunology
  • Pharmacology (medical)

Cite this

Shlopov, B. V., Lie, W. R., Mainardi, C. L., Cole, A. A., Chubinskaya, S., & Hasty, K. (1997). Osteoarthritic lesions: Involvement of three different collagenases. Arthritis and rheumatism, 40(11), 2065-2074. https://doi.org/10.1002/art.1780401120

Osteoarthritic lesions : Involvement of three different collagenases. / Shlopov, Boris V.; Lie, Wen Rong; Mainardi, Carlo L.; Cole, Ada A.; Chubinskaya, Susan; Hasty, Karen.

In: Arthritis and rheumatism, Vol. 40, No. 11, 01.11.1997, p. 2065-2074.

Research output: Contribution to journalArticle

Shlopov, BV, Lie, WR, Mainardi, CL, Cole, AA, Chubinskaya, S & Hasty, K 1997, 'Osteoarthritic lesions: Involvement of three different collagenases', Arthritis and rheumatism, vol. 40, no. 11, pp. 2065-2074. https://doi.org/10.1002/art.1780401120
Shlopov, Boris V. ; Lie, Wen Rong ; Mainardi, Carlo L. ; Cole, Ada A. ; Chubinskaya, Susan ; Hasty, Karen. / Osteoarthritic lesions : Involvement of three different collagenases. In: Arthritis and rheumatism. 1997 ; Vol. 40, No. 11. pp. 2065-2074.
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