Overexpression of pp60(c-src) elicits invasive behavior in rat colon epithelial cells

S. E. Pories, D. T. Hess, K. Swenson, M. Lotz, R. Moussa, Jr Steele G., David Shibata, K. M. Rieger-Christ, I. C. Summerhayes

Research output: Contribution to journalArticle

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Abstract

Background and Aims: Src activation is reported as an early event found in preneoplastic colonic adenomas and in 70% of colon carcinomas. The aim of this study was to identify the biological consequences of c-src overexpression in rat colon epithelial cells. Methods: Introduction and overexpression of c-src in an immortalized rat colon epithelial cell line was achieved using lipofection. Transfectants were tested for changes in growth and cell behavior using different in vitro assay systems. Results: Colon epithelial cells overexpressing c-src showed the ability to form microcolonies in soft agar without acquiring tumorigenic potential. In in vitro assays, c-src transfectants displayed a gain of invasive potential through Matrigel without an accompanying change in migrational ability. No discernible qualitative changes were observed in the phosphotyrosyl protein profile between c-src and v-src transfectants. Assessment of the cadherin/catenin status in these cells revealed an intact, functional complex with no detectable tyrosine phosphorylation of different components of the complex. Conclusions: Overexpression of c-src in an immortalized rat colon epithelial cell line does not elicit full neoplastic transformation but enhances anchorage-independent growth and confers invasion capability. Increased invasion through Matrigel was not linked to inactivation of the cadherin complex in c-src transfectants.

Original languageEnglish (US)
Pages (from-to)1287-1295
Number of pages9
JournalGastroenterology
Volume114
Issue number6
DOIs
StatePublished - Jan 1 1998

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Proto-Oncogene Proteins pp60(c-src)
Colon
Epithelial Cells
Aptitude
Cadherins
Cell Line
Catenins
Growth
Adenoma
Agar
Tyrosine
Phosphorylation
Carcinoma
Proteins

All Science Journal Classification (ASJC) codes

  • Gastroenterology

Cite this

Pories, S. E., Hess, D. T., Swenson, K., Lotz, M., Moussa, R., Steele G., J., ... Summerhayes, I. C. (1998). Overexpression of pp60(c-src) elicits invasive behavior in rat colon epithelial cells. Gastroenterology, 114(6), 1287-1295. https://doi.org/10.1016/S0016-5085(98)70435-4

Overexpression of pp60(c-src) elicits invasive behavior in rat colon epithelial cells. / Pories, S. E.; Hess, D. T.; Swenson, K.; Lotz, M.; Moussa, R.; Steele G., Jr; Shibata, David; Rieger-Christ, K. M.; Summerhayes, I. C.

In: Gastroenterology, Vol. 114, No. 6, 01.01.1998, p. 1287-1295.

Research output: Contribution to journalArticle

Pories, SE, Hess, DT, Swenson, K, Lotz, M, Moussa, R, Steele G., J, Shibata, D, Rieger-Christ, KM & Summerhayes, IC 1998, 'Overexpression of pp60(c-src) elicits invasive behavior in rat colon epithelial cells', Gastroenterology, vol. 114, no. 6, pp. 1287-1295. https://doi.org/10.1016/S0016-5085(98)70435-4
Pories SE, Hess DT, Swenson K, Lotz M, Moussa R, Steele G. J et al. Overexpression of pp60(c-src) elicits invasive behavior in rat colon epithelial cells. Gastroenterology. 1998 Jan 1;114(6):1287-1295. https://doi.org/10.1016/S0016-5085(98)70435-4
Pories, S. E. ; Hess, D. T. ; Swenson, K. ; Lotz, M. ; Moussa, R. ; Steele G., Jr ; Shibata, David ; Rieger-Christ, K. M. ; Summerhayes, I. C. / Overexpression of pp60(c-src) elicits invasive behavior in rat colon epithelial cells. In: Gastroenterology. 1998 ; Vol. 114, No. 6. pp. 1287-1295.
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