Overlapping positive and negative GATA factor binding sites mediate inducible DAL7 gene expression in Saccharomyces cerevisiae

Rajendra Rai, Jon R. Daugherty, Thomas S. Cunningham, Terrance Cooper

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

Allantoin pathway gene expression in Saccharomyces cerevisiae responds to two different environmental stimuli. The expression of these genes is induced in the presence of allantoin or its degradative metabolites and repressed when a good nitrogen source (e.g. asparagine or glutamine) is provided. Three types of cis-acting sites and trans-acting factors are required for allantoin pathway gene transcription as follows: (i) UAS(NTR) element associated with the transcriptional activators Gln3p and Gat1p, (ii) URS(GATA) element associated with the repressor Da180p, and (iii) UIS(ALL) element associated with the Da182 and Da181 proteins required for inducer- dependent transcription. Most of the work leading to the above conclusions has employed inducer-independent allantoin pathway genes (e.g. DAL5 and DAL3). The purpose of this work is to extend our understanding of these elements and their roles to inducible allantoin pathway genes using the DAL7 (encoding malate synthase) as a model. We show that eight distinct cis-acting sites participate in the process as follows: a newly identified GC-rich element, two UAS(NTR), two UIS(ALL), and three URS(GATA) elements. The two GATA-containing UAS(NTR) elements are coincident with two of the three GATA sequences that make up the URS(GATA) elements. The remaining URS(GATA) GATA sequence, however, is not a UAS(NTR) element but appears to function only in repression. The data provide insights into how these cis-and trans-acting factors function together to accomplish the regulated expression of the DAL7 gene that is observed in vivo.

Original languageEnglish (US)
Pages (from-to)28026-28034
Number of pages9
JournalJournal of Biological Chemistry
Volume274
Issue number39
DOIs
StatePublished - Sep 24 1999

Fingerprint

Allantoin
GATA Transcription Factors
Gene expression
Yeast
Saccharomyces cerevisiae
Genes
Binding Sites
Gene Expression
Trans-Activators
Transcription
Malate Synthase
Asparagine
Metabolites
Glutamine
Nitrogen
Proteins

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this

Overlapping positive and negative GATA factor binding sites mediate inducible DAL7 gene expression in Saccharomyces cerevisiae. / Rai, Rajendra; Daugherty, Jon R.; Cunningham, Thomas S.; Cooper, Terrance.

In: Journal of Biological Chemistry, Vol. 274, No. 39, 24.09.1999, p. 28026-28034.

Research output: Contribution to journalArticle

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