Oxidative metabolism

Glucose versus ketones

Allison Prince, Yifan Zhang, Colleen Croniger, Michelle Puchowicz

Research output: Contribution to journalArticle

Abstract

The coupling of upstream oxidative processes (glycolysis, beta-oxidation, CAC turnover) to mitochondrial oxidative phosphorylation (OXPHOS) under the driving conditions of energy demand by the cell results in the liberation of free energy as ATP. Perturbations in glycolytic CAC or OXPHOS can result in pathology or cell death. To better understand whole body energy expenditure during chronic ketosis, we used a diet-induced rat model of ketosis to determine if high-fat-carbohydrate-restricted "ketogenic" diet results in changes in total energy expenditure (TEE). Consistent with previous reports of increased energy expenditure in mice, we hypothesized that rats fed ketogenic diet for 3 weeks would result in increased resting energy expenditure due to alterations in metabolism associated with a "switch" in energy substrate from glucose to ketone bodies. The rationale is ketone bodies are a more efficient fuel than glucose. Indirect calorimetric analysis revealed a moderate increase in VO2 and decreased VCO2 and heat with ketosis. These results suggest ketosis induces a moderate uncoupling state and less oxidative efficiency compared to glucose oxidation.

Original languageEnglish (US)
Pages (from-to)323-328
Number of pages6
JournalAdvances in Experimental Medicine and Biology
Volume789
DOIs
StatePublished - 2013
Externally publishedYes

Fingerprint

Ketosis
Ketones
Metabolism
Energy Metabolism
Nutrition
Ketogenic Diet
Glucose
Ketone Bodies
Oxidative Phosphorylation
Rats
Oxidative Coupling
Carbohydrate-Restricted Diet
Mitochondrial Turnover
Oxidation
Pathology
Glycolysis
Cell death
Free energy
Cell Death
Hot Temperature

All Science Journal Classification (ASJC) codes

  • Biochemistry, Genetics and Molecular Biology(all)
  • Medicine(all)

Cite this

Oxidative metabolism : Glucose versus ketones. / Prince, Allison; Zhang, Yifan; Croniger, Colleen; Puchowicz, Michelle.

In: Advances in Experimental Medicine and Biology, Vol. 789, 2013, p. 323-328.

Research output: Contribution to journalArticle

Prince, Allison ; Zhang, Yifan ; Croniger, Colleen ; Puchowicz, Michelle. / Oxidative metabolism : Glucose versus ketones. In: Advances in Experimental Medicine and Biology. 2013 ; Vol. 789. pp. 323-328.
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