Oxidative stress and lipid retention in vascular grafts comparison between venous and arterial conduits

Yi Shi, Sachin Patel, Kelly L. Davenpeck, Rodica Niculescu, Evelio Rodriguez, Michael G. Magno, Michael L. Ormont, John D. Mannion, Andrew Zalewski

    Research output: Contribution to journalArticle

    49 Citations (Scopus)

    Abstract

    Background - Because saphenous vein grafts (SVGs) exhibit greater cellular heterogeneity and worse clinical outcomes than arterial grafts (AGs), we examined oxidative stress and lipid retention in different vascular conduits. Methods and Results - In a porcine model of graft interposition into carotid artery, superoxide anion (·O2 -) was measured at 2 weeks after surgery. SVGs demonstrated increased ·O2 - production compared with AGs (SOD-inhibitable nitro blue tetrazolium reduction, P<0.01). The NAD(P)H oxidase inhibitor diphenyleneiodonium (P<0.01) abolished SVG-derived ·O2 -, whereas the inhibitors of other pro-oxidant enzymes were ineffective. The change in oxidative stress was also reflected by lower activity of the endogenous antioxidant superoxide dismutase in SVGs than in AGs (P<0.001). SVG remodeling was associated with increased synthesis of sulfated glycosaminoglycans and augmented expression of a core protein, versican. These changes were accompanied by SVGs retaining significantly more 125I-labeled LDL than AGs ex vivo (P<0.001). In hyperlipemic animals, lipid accumulation and oxidized epitopes were preferentially noted in the intima of SVGs at 1 month after surgery. Conclusions - This study demonstrated significant differences in the biology of SVGs and AGs. SVGs exhibited higher oxidative stress, LDL accumulation, and the presence of oxidized epitopes. These findings suggest that proatherogenic changes in SVGs may commence early after surgical revasculanzatlon.

    Original languageEnglish (US)
    Pages (from-to)2408-2413
    Number of pages6
    JournalCirculation
    Volume103
    Issue number19
    DOIs
    StatePublished - May 15 2001

    Fingerprint

    Blood Vessels
    Oxidative Stress
    Saphenous Vein
    Transplants
    Lipids
    A73025
    Epitopes
    Versicans
    NADPH Oxidase
    Carotid Arteries
    Superoxides
    Superoxide Dismutase
    Reactive Oxygen Species
    Swine
    Antioxidants

    All Science Journal Classification (ASJC) codes

    • Cardiology and Cardiovascular Medicine
    • Physiology (medical)

    Cite this

    Shi, Y., Patel, S., Davenpeck, K. L., Niculescu, R., Rodriguez, E., Magno, M. G., ... Zalewski, A. (2001). Oxidative stress and lipid retention in vascular grafts comparison between venous and arterial conduits. Circulation, 103(19), 2408-2413. https://doi.org/10.1161/01.CIR.103.19.2408

    Oxidative stress and lipid retention in vascular grafts comparison between venous and arterial conduits. / Shi, Yi; Patel, Sachin; Davenpeck, Kelly L.; Niculescu, Rodica; Rodriguez, Evelio; Magno, Michael G.; Ormont, Michael L.; Mannion, John D.; Zalewski, Andrew.

    In: Circulation, Vol. 103, No. 19, 15.05.2001, p. 2408-2413.

    Research output: Contribution to journalArticle

    Shi, Y, Patel, S, Davenpeck, KL, Niculescu, R, Rodriguez, E, Magno, MG, Ormont, ML, Mannion, JD & Zalewski, A 2001, 'Oxidative stress and lipid retention in vascular grafts comparison between venous and arterial conduits', Circulation, vol. 103, no. 19, pp. 2408-2413. https://doi.org/10.1161/01.CIR.103.19.2408
    Shi Y, Patel S, Davenpeck KL, Niculescu R, Rodriguez E, Magno MG et al. Oxidative stress and lipid retention in vascular grafts comparison between venous and arterial conduits. Circulation. 2001 May 15;103(19):2408-2413. https://doi.org/10.1161/01.CIR.103.19.2408
    Shi, Yi ; Patel, Sachin ; Davenpeck, Kelly L. ; Niculescu, Rodica ; Rodriguez, Evelio ; Magno, Michael G. ; Ormont, Michael L. ; Mannion, John D. ; Zalewski, Andrew. / Oxidative stress and lipid retention in vascular grafts comparison between venous and arterial conduits. In: Circulation. 2001 ; Vol. 103, No. 19. pp. 2408-2413.
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    AU - Patel, Sachin

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    AU - Rodriguez, Evelio

    AU - Magno, Michael G.

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