Oxidative stress in the infarcted heart: Role of de novo angiotensin II production

Li Lu, Mark T. Quinn, Yao Sun

Research output: Contribution to journalArticle

40 Citations (Scopus)

Abstract

This study aimed to elucidate whether angiotensin (Ang) II generated de novo at the infarct site regulates the redox state of inflammatory cells participating in cardiac repair. On days 3-28 following ligation of the rat left coronary artery, we addressed at the infarct site: (a) the appearance and cellular origin of oxidative stress by monitoring the expression (mRNA and protein) of gp91phox (a subunit of superoxide producing NADPH oxidase) and the antioxidant superoxide dismutase (SOD), together with the presence of 3-nitrotyrosine (a marker of oxidative stress); (b) the presence of components requisite to Ang peptide generation; and (c) response to treatment with losartan (Los, 10 mg/kg/day). We found at the infarct site, macrophage-derived oxidative stress appears during week 1 coincident with the appearance of components requisite to AngII generation and activity in these cells. Based on observed response to AT1 receptor antagonism with Los, we would suggest de novo AngII, in an autocrine manner, participates in the induction of oxidative stress while suppressing the expression of antioxidant defenses.

Original languageEnglish (US)
Pages (from-to)943-951
Number of pages9
JournalBiochemical and Biophysical Research Communications
Volume325
Issue number3
DOIs
StatePublished - Dec 17 2004

Fingerprint

Oxidative stress
Angiotensin II
Oxidative Stress
Antioxidants
Losartan
Macrophages
NADPH Oxidase
Angiotensins
Superoxides
Superoxide Dismutase
Oxidation-Reduction
Ligation
Rats
Coronary Vessels
Repair
Messenger RNA
Peptides
Monitoring
Proteins

All Science Journal Classification (ASJC) codes

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this

Oxidative stress in the infarcted heart : Role of de novo angiotensin II production. / Lu, Li; Quinn, Mark T.; Sun, Yao.

In: Biochemical and Biophysical Research Communications, Vol. 325, No. 3, 17.12.2004, p. 943-951.

Research output: Contribution to journalArticle

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