p53 is involved in the p120E4F-mediated growth arrest

Peter Sandy, Monica Gostissa, Valentina Fogal, Loris De Cecco, Katalin Szalay, Robert Rooney, Claudio Schneider, Giannino Del Sal

Research output: Contribution to journalArticle

34 Citations (Scopus)

Abstract

Control of cell growth and division by the p53 tumor suppressor protein requires its abilities to transactivate and repress specific target genes and to associate in complex with other proteins. Here we demonstrate that p53 binds to the E1A-regulated transcription factor p120E4F, a transcriptional repressor of the adenovirus E4 promoter. The interaction involves carboxy-terminal half of p120E4F and sequences located at the end of the sequence-specific DNA-binding domain of p53. Ectopic expression of p120E4F leads to a block of cell proliferation in several human and murine cell lines and this effect requires the association with wild-type (wt) p53. Although p120E4F can also bind to mutant p53, the growth suppression induced by overexpression of the protein is severely reduced in a cell line that contains mutant p53. These data suggest that p120E4F may represent an important element within the complex network of p53 checkpoint functions.

Original languageEnglish (US)
Pages (from-to)188-199
Number of pages12
JournalOncogene
Volume19
Issue number2
DOIs
StatePublished - Jan 13 2000

Fingerprint

Tumor Suppressor Protein p53
Cell Line
Growth
Adenoviridae
Cell Division
Proteins
Cell Proliferation
Genes
transcription factor p120(E4F)
Ectopic Gene Expression

All Science Journal Classification (ASJC) codes

  • Molecular Biology
  • Genetics
  • Cancer Research

Cite this

Sandy, P., Gostissa, M., Fogal, V., De Cecco, L., Szalay, K., Rooney, R., ... Del Sal, G. (2000). p53 is involved in the p120E4F-mediated growth arrest. Oncogene, 19(2), 188-199. https://doi.org/10.1038/sj.onc.1203250

p53 is involved in the p120E4F-mediated growth arrest. / Sandy, Peter; Gostissa, Monica; Fogal, Valentina; De Cecco, Loris; Szalay, Katalin; Rooney, Robert; Schneider, Claudio; Del Sal, Giannino.

In: Oncogene, Vol. 19, No. 2, 13.01.2000, p. 188-199.

Research output: Contribution to journalArticle

Sandy, P, Gostissa, M, Fogal, V, De Cecco, L, Szalay, K, Rooney, R, Schneider, C & Del Sal, G 2000, 'p53 is involved in the p120E4F-mediated growth arrest', Oncogene, vol. 19, no. 2, pp. 188-199. https://doi.org/10.1038/sj.onc.1203250
Sandy P, Gostissa M, Fogal V, De Cecco L, Szalay K, Rooney R et al. p53 is involved in the p120E4F-mediated growth arrest. Oncogene. 2000 Jan 13;19(2):188-199. https://doi.org/10.1038/sj.onc.1203250
Sandy, Peter ; Gostissa, Monica ; Fogal, Valentina ; De Cecco, Loris ; Szalay, Katalin ; Rooney, Robert ; Schneider, Claudio ; Del Sal, Giannino. / p53 is involved in the p120E4F-mediated growth arrest. In: Oncogene. 2000 ; Vol. 19, No. 2. pp. 188-199.
@article{f95b52998eff4c50bb5c74e8df18a60e,
title = "p53 is involved in the p120E4F-mediated growth arrest",
abstract = "Control of cell growth and division by the p53 tumor suppressor protein requires its abilities to transactivate and repress specific target genes and to associate in complex with other proteins. Here we demonstrate that p53 binds to the E1A-regulated transcription factor p120E4F, a transcriptional repressor of the adenovirus E4 promoter. The interaction involves carboxy-terminal half of p120E4F and sequences located at the end of the sequence-specific DNA-binding domain of p53. Ectopic expression of p120E4F leads to a block of cell proliferation in several human and murine cell lines and this effect requires the association with wild-type (wt) p53. Although p120E4F can also bind to mutant p53, the growth suppression induced by overexpression of the protein is severely reduced in a cell line that contains mutant p53. These data suggest that p120E4F may represent an important element within the complex network of p53 checkpoint functions.",
author = "Peter Sandy and Monica Gostissa and Valentina Fogal and {De Cecco}, Loris and Katalin Szalay and Robert Rooney and Claudio Schneider and {Del Sal}, Giannino",
year = "2000",
month = "1",
day = "13",
doi = "10.1038/sj.onc.1203250",
language = "English (US)",
volume = "19",
pages = "188--199",
journal = "Oncogene",
issn = "0950-9232",
publisher = "Nature Publishing Group",
number = "2",

}

TY - JOUR

T1 - p53 is involved in the p120E4F-mediated growth arrest

AU - Sandy, Peter

AU - Gostissa, Monica

AU - Fogal, Valentina

AU - De Cecco, Loris

AU - Szalay, Katalin

AU - Rooney, Robert

AU - Schneider, Claudio

AU - Del Sal, Giannino

PY - 2000/1/13

Y1 - 2000/1/13

N2 - Control of cell growth and division by the p53 tumor suppressor protein requires its abilities to transactivate and repress specific target genes and to associate in complex with other proteins. Here we demonstrate that p53 binds to the E1A-regulated transcription factor p120E4F, a transcriptional repressor of the adenovirus E4 promoter. The interaction involves carboxy-terminal half of p120E4F and sequences located at the end of the sequence-specific DNA-binding domain of p53. Ectopic expression of p120E4F leads to a block of cell proliferation in several human and murine cell lines and this effect requires the association with wild-type (wt) p53. Although p120E4F can also bind to mutant p53, the growth suppression induced by overexpression of the protein is severely reduced in a cell line that contains mutant p53. These data suggest that p120E4F may represent an important element within the complex network of p53 checkpoint functions.

AB - Control of cell growth and division by the p53 tumor suppressor protein requires its abilities to transactivate and repress specific target genes and to associate in complex with other proteins. Here we demonstrate that p53 binds to the E1A-regulated transcription factor p120E4F, a transcriptional repressor of the adenovirus E4 promoter. The interaction involves carboxy-terminal half of p120E4F and sequences located at the end of the sequence-specific DNA-binding domain of p53. Ectopic expression of p120E4F leads to a block of cell proliferation in several human and murine cell lines and this effect requires the association with wild-type (wt) p53. Although p120E4F can also bind to mutant p53, the growth suppression induced by overexpression of the protein is severely reduced in a cell line that contains mutant p53. These data suggest that p120E4F may represent an important element within the complex network of p53 checkpoint functions.

UR - http://www.scopus.com/inward/record.url?scp=0034642488&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0034642488&partnerID=8YFLogxK

U2 - 10.1038/sj.onc.1203250

DO - 10.1038/sj.onc.1203250

M3 - Article

VL - 19

SP - 188

EP - 199

JO - Oncogene

JF - Oncogene

SN - 0950-9232

IS - 2

ER -