Pancreatic D-cell disorder in Coxsackievirus B4-induced diabetic mice

Nando K. Chatterjee, Ivan Gerling, Catherine Nejman

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Pancreatic D-cell disorder was analyzed in Coxsackievirus B4-induced diabetic mice employing molecular hybridization with a radiolabelled probe to quantitate somatostatin mRNA, and specific immunoprecipitation to measure somatostatin synthesis and its release. Many infected mice showed blood glucose lower than noninfected control animals at 72 h postinfection and 85% became hyperglycemic at 6-8 weeks postinfection. Pancreatic somatostatin decreased by 24% and 43% at 72 h and 6 weeks postinfection, respectively, while somatostatin release in islets from the infected mice increased by 2-fold or more. Residual islet somatostatin content after release was initially higher than control at 72 h and then declined at 6 weeks. Islet cellular RNA content decreased by 35% at 6 weeks, somatostatin mRNA content decreased by approximately 45% at 72 h and 6 weeks postinfection. D-cell disorder - somatostatin mRNA supply, synthesis, and release - is clearly evident in this model, which could be of significance in type I diabetes.

Original languageEnglish (US)
Pages (from-to)39-45
Number of pages7
JournalMolecular and Cellular Endocrinology
Volume67
Issue number1
DOIs
StatePublished - Jan 1 1989

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Somatostatin-Secreting Cells
Enterovirus
Somatostatin
Messenger RNA
Medical problems
Type 1 Diabetes Mellitus
Immunoprecipitation
Blood Glucose
Animals
RNA

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Molecular Biology
  • Endocrinology

Cite this

Pancreatic D-cell disorder in Coxsackievirus B4-induced diabetic mice. / Chatterjee, Nando K.; Gerling, Ivan; Nejman, Catherine.

In: Molecular and Cellular Endocrinology, Vol. 67, No. 1, 01.01.1989, p. 39-45.

Research output: Contribution to journalArticle

Chatterjee, Nando K. ; Gerling, Ivan ; Nejman, Catherine. / Pancreatic D-cell disorder in Coxsackievirus B4-induced diabetic mice. In: Molecular and Cellular Endocrinology. 1989 ; Vol. 67, No. 1. pp. 39-45.
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