Parasympathetic innervation of the meibomian glands in rats

Mark Ledoux, Q. Zhou, R. B. Murphy, M. L. Greene, P. Ryan

Research output: Contribution to journalArticle

39 Citations (Scopus)

Abstract

PURPOSE. To determine the location of parasympathetic neurons that innervate the meibomian glands in rats. METHODS. The B subunit of cholera toxin (CTB), fast blue, and a retrograde transneuronal tracer, the Bartha strain of pseudorabies virus (PRV-Ba), were injected into the upper eyelids of adult Sprague-Dawley rats after sectioning the ipsilateral branches of the facial nerve and resecting the superior cervical ganglia. Brains and orbital tissues were processed for the immunohistochemical detection of PRV-Ba and CTB. In selected cases, series of brain sections were double labeled for PRV-Ba and tyrosine hydroxylase to determine the relationship between the A5 noradrenergic cell group and superior salivatory nucleus, or for PRV-Ba and choline acetyltransferase to establish the neurochemical phenotype of parasympathetic preganglionic neurons. RESULTS. Labeled ganglionic cells were diffusely distributed within the ipsilateral pterygopalatine ganglion (PPG) and along the more proximal portions of the greater petrosal nerve (GPN). Labeled preganglionic neurons were cholinergic and were located immediately dorsolateral to the rostral-most portion of the facial nucleus and caudal superior olive, where they intermingled with A5 noradrenergic cells. CONCLUSIONS. The meibomian glands and other structures within the lid margin are subject to parasympathetic regulation by ganglion cells diffusely distributed within the PPG and along more proximal portions of the GPN. Cholinergic parasympathetic preganglionic neurons that project to meibomian gland-innervating ganglion cells are located immediately lateral, dorsal, and rostral to the facial motor nucleus in the region commonly referred to as the superior salivatory nucleus.

Original languageEnglish (US)
Pages (from-to)2434-2441
Number of pages8
JournalInvestigative Ophthalmology and Visual Science
Volume42
Issue number11
StatePublished - Oct 22 2001

Fingerprint

Meibomian Glands
Parasympathetic Ganglia
Neurons
Suid Herpesvirus 1
Superior Cervical Ganglion
Cholinergic Neurons
Choline O-Acetyltransferase
Cholera Toxin
Facial Nerve
Brain
Tyrosine 3-Monooxygenase
Eyelids
Ganglia
Cholinergic Agents
Sprague Dawley Rats
Phenotype

All Science Journal Classification (ASJC) codes

  • Ophthalmology
  • Sensory Systems
  • Cellular and Molecular Neuroscience

Cite this

Ledoux, M., Zhou, Q., Murphy, R. B., Greene, M. L., & Ryan, P. (2001). Parasympathetic innervation of the meibomian glands in rats. Investigative Ophthalmology and Visual Science, 42(11), 2434-2441.

Parasympathetic innervation of the meibomian glands in rats. / Ledoux, Mark; Zhou, Q.; Murphy, R. B.; Greene, M. L.; Ryan, P.

In: Investigative Ophthalmology and Visual Science, Vol. 42, No. 11, 22.10.2001, p. 2434-2441.

Research output: Contribution to journalArticle

Ledoux, M, Zhou, Q, Murphy, RB, Greene, ML & Ryan, P 2001, 'Parasympathetic innervation of the meibomian glands in rats', Investigative Ophthalmology and Visual Science, vol. 42, no. 11, pp. 2434-2441.
Ledoux, Mark ; Zhou, Q. ; Murphy, R. B. ; Greene, M. L. ; Ryan, P. / Parasympathetic innervation of the meibomian glands in rats. In: Investigative Ophthalmology and Visual Science. 2001 ; Vol. 42, No. 11. pp. 2434-2441.
@article{ae5e32266cff4e1b81e93c76f87388cb,
title = "Parasympathetic innervation of the meibomian glands in rats",
abstract = "PURPOSE. To determine the location of parasympathetic neurons that innervate the meibomian glands in rats. METHODS. The B subunit of cholera toxin (CTB), fast blue, and a retrograde transneuronal tracer, the Bartha strain of pseudorabies virus (PRV-Ba), were injected into the upper eyelids of adult Sprague-Dawley rats after sectioning the ipsilateral branches of the facial nerve and resecting the superior cervical ganglia. Brains and orbital tissues were processed for the immunohistochemical detection of PRV-Ba and CTB. In selected cases, series of brain sections were double labeled for PRV-Ba and tyrosine hydroxylase to determine the relationship between the A5 noradrenergic cell group and superior salivatory nucleus, or for PRV-Ba and choline acetyltransferase to establish the neurochemical phenotype of parasympathetic preganglionic neurons. RESULTS. Labeled ganglionic cells were diffusely distributed within the ipsilateral pterygopalatine ganglion (PPG) and along the more proximal portions of the greater petrosal nerve (GPN). Labeled preganglionic neurons were cholinergic and were located immediately dorsolateral to the rostral-most portion of the facial nucleus and caudal superior olive, where they intermingled with A5 noradrenergic cells. CONCLUSIONS. The meibomian glands and other structures within the lid margin are subject to parasympathetic regulation by ganglion cells diffusely distributed within the PPG and along more proximal portions of the GPN. Cholinergic parasympathetic preganglionic neurons that project to meibomian gland-innervating ganglion cells are located immediately lateral, dorsal, and rostral to the facial motor nucleus in the region commonly referred to as the superior salivatory nucleus.",
author = "Mark Ledoux and Q. Zhou and Murphy, {R. B.} and Greene, {M. L.} and P. Ryan",
year = "2001",
month = "10",
day = "22",
language = "English (US)",
volume = "42",
pages = "2434--2441",
journal = "Investigative Ophthalmology and Visual Science",
issn = "0146-0404",
publisher = "Association for Research in Vision and Ophthalmology Inc.",
number = "11",

}

TY - JOUR

T1 - Parasympathetic innervation of the meibomian glands in rats

AU - Ledoux, Mark

AU - Zhou, Q.

AU - Murphy, R. B.

AU - Greene, M. L.

AU - Ryan, P.

PY - 2001/10/22

Y1 - 2001/10/22

N2 - PURPOSE. To determine the location of parasympathetic neurons that innervate the meibomian glands in rats. METHODS. The B subunit of cholera toxin (CTB), fast blue, and a retrograde transneuronal tracer, the Bartha strain of pseudorabies virus (PRV-Ba), were injected into the upper eyelids of adult Sprague-Dawley rats after sectioning the ipsilateral branches of the facial nerve and resecting the superior cervical ganglia. Brains and orbital tissues were processed for the immunohistochemical detection of PRV-Ba and CTB. In selected cases, series of brain sections were double labeled for PRV-Ba and tyrosine hydroxylase to determine the relationship between the A5 noradrenergic cell group and superior salivatory nucleus, or for PRV-Ba and choline acetyltransferase to establish the neurochemical phenotype of parasympathetic preganglionic neurons. RESULTS. Labeled ganglionic cells were diffusely distributed within the ipsilateral pterygopalatine ganglion (PPG) and along the more proximal portions of the greater petrosal nerve (GPN). Labeled preganglionic neurons were cholinergic and were located immediately dorsolateral to the rostral-most portion of the facial nucleus and caudal superior olive, where they intermingled with A5 noradrenergic cells. CONCLUSIONS. The meibomian glands and other structures within the lid margin are subject to parasympathetic regulation by ganglion cells diffusely distributed within the PPG and along more proximal portions of the GPN. Cholinergic parasympathetic preganglionic neurons that project to meibomian gland-innervating ganglion cells are located immediately lateral, dorsal, and rostral to the facial motor nucleus in the region commonly referred to as the superior salivatory nucleus.

AB - PURPOSE. To determine the location of parasympathetic neurons that innervate the meibomian glands in rats. METHODS. The B subunit of cholera toxin (CTB), fast blue, and a retrograde transneuronal tracer, the Bartha strain of pseudorabies virus (PRV-Ba), were injected into the upper eyelids of adult Sprague-Dawley rats after sectioning the ipsilateral branches of the facial nerve and resecting the superior cervical ganglia. Brains and orbital tissues were processed for the immunohistochemical detection of PRV-Ba and CTB. In selected cases, series of brain sections were double labeled for PRV-Ba and tyrosine hydroxylase to determine the relationship between the A5 noradrenergic cell group and superior salivatory nucleus, or for PRV-Ba and choline acetyltransferase to establish the neurochemical phenotype of parasympathetic preganglionic neurons. RESULTS. Labeled ganglionic cells were diffusely distributed within the ipsilateral pterygopalatine ganglion (PPG) and along the more proximal portions of the greater petrosal nerve (GPN). Labeled preganglionic neurons were cholinergic and were located immediately dorsolateral to the rostral-most portion of the facial nucleus and caudal superior olive, where they intermingled with A5 noradrenergic cells. CONCLUSIONS. The meibomian glands and other structures within the lid margin are subject to parasympathetic regulation by ganglion cells diffusely distributed within the PPG and along more proximal portions of the GPN. Cholinergic parasympathetic preganglionic neurons that project to meibomian gland-innervating ganglion cells are located immediately lateral, dorsal, and rostral to the facial motor nucleus in the region commonly referred to as the superior salivatory nucleus.

UR - http://www.scopus.com/inward/record.url?scp=0034788086&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0034788086&partnerID=8YFLogxK

M3 - Article

VL - 42

SP - 2434

EP - 2441

JO - Investigative Ophthalmology and Visual Science

JF - Investigative Ophthalmology and Visual Science

SN - 0146-0404

IS - 11

ER -