Participation of ABF-1 protein in expression of the saccharomyces cerevisiae CAR1 gene

L. Z. Kovari, Terrance Cooper

Research output: Contribution to journalArticle

24 Citations (Scopus)

Abstract

DNA fragments previously shown to be required for expression of the CAR1 (arginase) gene in Saccharomyces cerevisiae and to support transcriptional activation of a reporter gene in a heterologous expression vector were shown to bind purified regulatory protein ABF-1. Two ABF-1 sites were identified in the CAR1 upstream region, one to which ABF-1 protein bound with high affinity and a second to which it bound much less avidly. The higher-affinity ABF-1 binding site upstream of CAR1 was an effective competitor of the HMRE, ARS1 B domain, and COR2-GFI binding sequences for protein binding. Point mutations in the CAR1 high-affinity ABF-1 binding site resulted in a 12-fold loss of transcriptional activation of a reporter gene compared with the wild-type CAR1 DNA fragment. These data are consistent with the suggestion that ABF-1 protein is one of the transcription factors involved in expression of the CAR1 gene.

Original languageEnglish (US)
Pages (from-to)6332-6338
Number of pages7
JournalJournal of bacteriology
Volume173
Issue number20
DOIs
StatePublished - Jan 1 1991

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Saccharomyces cerevisiae
Reporter Genes
Transcriptional Activation
Binding Sites
Genes
Arginase
Proteins
DNA
Point Mutation
Protein Binding
Carrier Proteins
Transcription Factors
Gene Expression

All Science Journal Classification (ASJC) codes

  • Microbiology
  • Molecular Biology

Cite this

Participation of ABF-1 protein in expression of the saccharomyces cerevisiae CAR1 gene. / Kovari, L. Z.; Cooper, Terrance.

In: Journal of bacteriology, Vol. 173, No. 20, 01.01.1991, p. 6332-6338.

Research output: Contribution to journalArticle

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