Participation of RAP1 protein in expression of the Saccharomyces cerevisiae arginase (CAR1) gene

L. Z. Kovari, I. Kovari, Terrance Cooper

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

Regulated expression of the inducible arginase (CAR1) gene of Saccharomyces cerevisiae has been shown to require three upstream activation sequences (UASs) and an upstream repression sequence, URS1. Two of the UAS elements, UAS(C1) and UAS(C2), operate in an inducer-independent manner, while the third, UAS(I), is inducer dependent. UAS(C1) and UAS(C2) were previously shown to contain ABF-1 binding sites that were required for normal transcription. In this work, we demonstrate that UAS(C1) and UAS(C2) also contain two and three sites, respectively, that are able to bind RAP1 protein. RAP1 binding to these sites, however, is significantly weaker than that to sites in TEF2 and HMRE. The effects of mutating the sites individually or in combination suggest that at least three of them, two in UAS(C1) and one in UAS(C2), probably participate in CAR1 expression.

Original languageEnglish (US)
Pages (from-to)941-951
Number of pages11
JournalJournal of Bacteriology
Volume175
Issue number4
DOIs
StatePublished - Jan 1 1993

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Arginase
Saccharomyces cerevisiae
Binding Sites
Genes
Proteins

All Science Journal Classification (ASJC) codes

  • Microbiology
  • Molecular Biology

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Participation of RAP1 protein in expression of the Saccharomyces cerevisiae arginase (CAR1) gene. / Kovari, L. Z.; Kovari, I.; Cooper, Terrance.

In: Journal of Bacteriology, Vol. 175, No. 4, 01.01.1993, p. 941-951.

Research output: Contribution to journalArticle

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