Passive immunization with antiserum to a nontoxic alpha-toxin mutant from Staphylococcus aureus is protective in a murine model

Barbara Menzies, Douglas S. Kernodle

Research output: Contribution to journalArticle

69 Citations (Scopus)

Abstract

A nonhemolytic, nonlethal variant of Staphylococcus aureus alpha-toxin constructed via oligonucleotide-directed mutagenesis and containing a single amino acid substitution (H-35 to L) was used to immunize a rabbit. The resulting antiserum was cross-reactive with wild-type alpha-toxin and neutralized its hemolytic activity in vitro. Passive immunization of mice with rabbit antiserum conferred protection against lethal challenge with wild-type alpha-toxin and against acute lethal challenge with a high-alpha- toxin-producing S. aureus strain. H35L alpha-toxin may be useful as a protective immunogen in S. aureus vaccine studies.

Original languageEnglish (US)
Pages (from-to)1839-1841
Number of pages3
JournalInfection and Immunity
Volume64
Issue number5
StatePublished - May 9 1996
Externally publishedYes

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Passive Immunization
Immune Sera
Rabbits
Amino Acid Substitution
Site-Directed Mutagenesis
Staphylococcus aureus
Vaccines
staphylococcal alpha-toxin
In Vitro Techniques

All Science Journal Classification (ASJC) codes

  • Parasitology
  • Microbiology
  • Immunology
  • Infectious Diseases

Cite this

Passive immunization with antiserum to a nontoxic alpha-toxin mutant from Staphylococcus aureus is protective in a murine model. / Menzies, Barbara; Kernodle, Douglas S.

In: Infection and Immunity, Vol. 64, No. 5, 09.05.1996, p. 1839-1841.

Research output: Contribution to journalArticle

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