Passive spatial and temporal integration of excitatory synaptic inputs in cerebellar Purkinje cells of young rats

Detlef Heck, Alexander Borst, Bernd Antkowiak

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Abstract

We have investigated the integration of excitatory (parallel fiber) synaptic inputs in cerebellar Purkinje cells of young rats in vitro and in a compartmental model of such a cell, based on 3D morphological reconstruction. Excitatory synaptic inputs at two independent dendritic sites were activated by electrical stimulation with various delays between the two stimuli. Population postsynaptic potentials summed linearly under current clamp condition when the two dendritic input sites were spatially separated (>200 μm) but sublinearly, in a delay dependent manner, when the input sites were close (<50 μm) to each other. Population postsynaptic currents measured under voltage clamp conditions summed linearly independent of the spatial and temporal separation of inputs. Summation of inputs in a passive compartmental model of a Purkinje cell was similar to that of Purkinje cells in vitro. We show that sublinear summation of neighboring inputs is independent of inhibitory mechanisms and suggest that sublinearity is mainly due to a locally reduced driving force.

Original languageEnglish (US)
Pages (from-to)79-83
Number of pages5
JournalNeuroscience Letters
Volume341
Issue number1
DOIs
StatePublished - Apr 24 2003
Externally publishedYes

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Purkinje Cells
Synaptic Potentials
Population
Electric Stimulation
In Vitro Techniques

All Science Journal Classification (ASJC) codes

  • Neuroscience(all)

Cite this

Passive spatial and temporal integration of excitatory synaptic inputs in cerebellar Purkinje cells of young rats. / Heck, Detlef; Borst, Alexander; Antkowiak, Bernd.

In: Neuroscience Letters, Vol. 341, No. 1, 24.04.2003, p. 79-83.

Research output: Contribution to journalArticle

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