Pathology of AA amyloidosis in domestic sheep and goats

C. Ménsua, L. Carrasco, M. J. Bautista, E. Biescas, A. Fernández, C. L. Murphy, D. T. Weiss, Alan Solomon, L. Luján

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Abstract

We describe the main pathologic changes in small ruminants affected by AA amyloidosis, together with the partial sequence of the protein involved. Twenty-one sheep and one goat were selected for presenting macroscopic kidney lesions compatible with systemic amyloidosis. Available tissue samples were studied by histologic, immunopathologic, and ultrastructural means. Renal lesions were characterized grossly by pale cortical surfaces with scattered, miliary, whitish-yellow foci and on cut cortical surfaces by straight, whitish-yellow striations. Gangrenous pneumonia was observed in 16 out of 21 affected sheep (76.2%), although other chronic inflammations were also observed. Amyloid was detected in all grossly affected kidneys using Congo red staining, lesions being most remarkable in glomeruli, affecting 95.5% of animals studied. Congophilic deposits were also observed in intertubular interstitium (68.2%) and medulla (57.1%). All amyloid-affected animals presented proximal convoluted tubule lesions, mostly characterized by an increase in diameter and by hyaline granular degeneration that were responsible for the macroscopic appearance of the kidney. Histologically, amyloid was also seen in blood vessels, spleen, liver, lymph nodes, gastrointestinal tract, and adrenal glands. All amyloid deposits demonstrated greenish-yellow birefringence with polarized light, and the antisera prepared against goat amyloid extracts specifically reacted with birefringent congophilic deposits of both sheep and goats. Ultrastructurally, these deposits were formed by masses of straight, nonbranching fibrils located predominantly in the basement membranes of glomerular capillaries and in the mesangium. Partial sequence of the protein in sheep and goats indicated a high degree of homology with the previously reported sequence of sheep Serum Amyloid A.

Original languageEnglish (US)
Pages (from-to)71-80
Number of pages10
JournalVeterinary Pathology
Volume40
Issue number1
DOIs
StatePublished - Jan 1 2003

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Domestic Sheep
amyloidosis
Amyloidosis
amyloid
Goats
Sheep
Amyloid
goats
Pathology
sheep
lesions (animal)
Kidney
kidneys
Birefringence
Serum Amyloid A Protein
amino acid sequences
Congo Red
Glomerular Basement Membrane
Hyalin
Amyloid Plaques

All Science Journal Classification (ASJC) codes

  • veterinary(all)

Cite this

Ménsua, C., Carrasco, L., Bautista, M. J., Biescas, E., Fernández, A., Murphy, C. L., ... Luján, L. (2003). Pathology of AA amyloidosis in domestic sheep and goats. Veterinary Pathology, 40(1), 71-80. https://doi.org/10.1354/vp.40-1-71

Pathology of AA amyloidosis in domestic sheep and goats. / Ménsua, C.; Carrasco, L.; Bautista, M. J.; Biescas, E.; Fernández, A.; Murphy, C. L.; Weiss, D. T.; Solomon, Alan; Luján, L.

In: Veterinary Pathology, Vol. 40, No. 1, 01.01.2003, p. 71-80.

Research output: Contribution to journalArticle

Ménsua, C, Carrasco, L, Bautista, MJ, Biescas, E, Fernández, A, Murphy, CL, Weiss, DT, Solomon, A & Luján, L 2003, 'Pathology of AA amyloidosis in domestic sheep and goats', Veterinary Pathology, vol. 40, no. 1, pp. 71-80. https://doi.org/10.1354/vp.40-1-71
Ménsua C, Carrasco L, Bautista MJ, Biescas E, Fernández A, Murphy CL et al. Pathology of AA amyloidosis in domestic sheep and goats. Veterinary Pathology. 2003 Jan 1;40(1):71-80. https://doi.org/10.1354/vp.40-1-71
Ménsua, C. ; Carrasco, L. ; Bautista, M. J. ; Biescas, E. ; Fernández, A. ; Murphy, C. L. ; Weiss, D. T. ; Solomon, Alan ; Luján, L. / Pathology of AA amyloidosis in domestic sheep and goats. In: Veterinary Pathology. 2003 ; Vol. 40, No. 1. pp. 71-80.
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