PECAM-1/CD31 expression on brain microvessel endothelium and T cells

M. Kim-Miller, Z. Radvany, M. Sandor, Francesca-Fang Liao, W. A. Muller, Z. Fabry

Research output: Contribution to journalArticle

Abstract

Infiltration of CD4+ T cells into the brain is thought to be an important step in the initiation of CNS autoimmune diseases. The role of adhesion molecules in the migration of antigen-specific T cells is unclear. Platelet/endothelial cell adhesion molecule-1 (PECAM-1/CD31) is involved in monocyte and neutrophil transendothelial migration, but its role in antigen(Ag)-specific T cell transendothelial migration is unknown. We examined CD31 expression on brain microvessel endothelial (En) cells and T cells and its regulation by inflammatory cytokines. FACS analysis revealed constitutive CD31 expression on murine brain microvessel En cells. When the cells were stimulated using cytokines IL-1α, TNF-α or IFN-γ for 24 to 72 hrs, CD31 was upregulated by IFN-γ and TNF-α. Using a fusion protein, PECAM-1/Ig, expression of CD31 ligand on the same En cells was also examined. We found that the ligand was upregulated by IL-1α, IFN-γ and TNF-α. CD31 is also upregulated on α/β TCR+ T cells. Ag-specific T cells were generated using PCC peptide-specific AND α/β TCR and G8 T10b alloantigenic γ/δ TCR transgenic mice. FACS analysis revealed that Ag-specific activation of α/β TCR+ T cells produced progressive CD31 upregulation over 72 hrs whereas nonspecific activation resulted in faster but less upregulation overall. CD31 on γ/δ TCR+ T cells was unaltered. Lastly, we demonstrated localization of CD31 to contact sites between activated T cells and brain microvessel En cells. These data further suggest an important role for CD31 on brain microvessel En cells and T cells in the regulation of CNS inflammatory events.

Original languageEnglish (US)
JournalFASEB Journal
Volume12
Issue number5
StatePublished - Mar 20 1998
Externally publishedYes

Fingerprint

CD31 Antigens
T-cells
Microvessels
Endothelium
Brain
T-Lymphocytes
Endothelial cells
Endothelial Cells
Transendothelial and Transepithelial Migration
Antigens
Interleukin-1
Up-Regulation
Chemical activation
Cytokines
Ligands
Central Nervous System Diseases
Infiltration
Transgenic Mice
Autoimmune Diseases
Cell Movement

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Biochemistry
  • Molecular Biology
  • Genetics

Cite this

Kim-Miller, M., Radvany, Z., Sandor, M., Liao, F-F., Muller, W. A., & Fabry, Z. (1998). PECAM-1/CD31 expression on brain microvessel endothelium and T cells. FASEB Journal, 12(5).

PECAM-1/CD31 expression on brain microvessel endothelium and T cells. / Kim-Miller, M.; Radvany, Z.; Sandor, M.; Liao, Francesca-Fang; Muller, W. A.; Fabry, Z.

In: FASEB Journal, Vol. 12, No. 5, 20.03.1998.

Research output: Contribution to journalArticle

Kim-Miller, M, Radvany, Z, Sandor, M, Liao, F-F, Muller, WA & Fabry, Z 1998, 'PECAM-1/CD31 expression on brain microvessel endothelium and T cells', FASEB Journal, vol. 12, no. 5.
Kim-Miller M, Radvany Z, Sandor M, Liao F-F, Muller WA, Fabry Z. PECAM-1/CD31 expression on brain microvessel endothelium and T cells. FASEB Journal. 1998 Mar 20;12(5).
Kim-Miller, M. ; Radvany, Z. ; Sandor, M. ; Liao, Francesca-Fang ; Muller, W. A. ; Fabry, Z. / PECAM-1/CD31 expression on brain microvessel endothelium and T cells. In: FASEB Journal. 1998 ; Vol. 12, No. 5.
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