PEG-functionalized magnetic nanoparticles for drug delivery and magnetic resonance imaging applications

Murali Yallapu, Susan P. Foy, Tapan K. Jain, Vinod Labhasetwar

Research output: Contribution to journalArticle

101 Citations (Scopus)

Abstract

Purpose: Polyethylene glycol (PEG) functionalized magnetic nanoparticles (MNPs) were tested as a drug carrier system, as a magnetic resonance imaging (MRI) agent, and for their ability to conjugate to an antibody. Methods: An iron oxide core coated with oleic acid (OA) and then with OA-PEG forms a water-dispersible MNP formulation. Hydrophobic doxorubicin partitions into the OA layer for sustained drug delivery. The T1 and T2 MRI contrast properties were determined in vitro and the circulation of the MNPs was measured in mouse carotid arteries. An N-hydroxysuccinimide group (NHS) on the OA-PEG-80 was used to conjugate the amine functional group on antibodies for active targeting in the human MCF-7 breast cancer cell line. Results: The optimized formulation had a mean hydrodynamic diameter of 184 nm with an ∼8 nm iron-oxide core. The MNPs enhance the T2 MRI contrast and have a long circulation time in vivo with 30% relative concentration 50 min post-injection. Doxorubicin-loaded MNPs showed sustained drug release and dose-dependent antiproliferative effects in vitro; the drug effect was enhanced with transferrin antibody-conjugated MNPs. Conclusion: PEG-functionalized MNPs could be developed as a targeted drug delivery system and MRI contrast agent.

Original languageEnglish (US)
Pages (from-to)2283-2295
Number of pages13
JournalPharmaceutical Research
Volume27
Issue number11
DOIs
StatePublished - Nov 1 2010
Externally publishedYes

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Magnetic resonance
Drug delivery
Nanoparticles
Magnetic Resonance Imaging
Imaging techniques
Oleic Acid
Pharmaceutical Preparations
Doxorubicin
Antibodies
Drug Carriers
Hydrodynamics
Drug Delivery Systems
Transferrin
Carotid Arteries
Contrast Media
Functional groups
Amines
Cells
Breast Neoplasms
Cell Line

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Molecular Medicine
  • Pharmacology
  • Pharmaceutical Science
  • Organic Chemistry
  • Pharmacology (medical)

Cite this

PEG-functionalized magnetic nanoparticles for drug delivery and magnetic resonance imaging applications. / Yallapu, Murali; Foy, Susan P.; Jain, Tapan K.; Labhasetwar, Vinod.

In: Pharmaceutical Research, Vol. 27, No. 11, 01.11.2010, p. 2283-2295.

Research output: Contribution to journalArticle

Yallapu, Murali ; Foy, Susan P. ; Jain, Tapan K. ; Labhasetwar, Vinod. / PEG-functionalized magnetic nanoparticles for drug delivery and magnetic resonance imaging applications. In: Pharmaceutical Research. 2010 ; Vol. 27, No. 11. pp. 2283-2295.
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