Pentobarbital differentially enhances the affinity of [3h]flunitrazepam binding across brain regions

Berit X. Carlson, Helen Baghdoyan

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

The present study used saturation binding analyses to test the hypothesis that pentobarbital would differentially increase the affinity of benzodiazepine binding across brain regions. The results showed that there were significant (p < 0.05) regional differences in the dissociation constant (KD) for tritiated fluni- trazepam ([3H]FLU) and that pentobarbital differentially decreased the Kd for [3H]FLU across 6 brain regions. Pentobarbital caused the greatest decrease (−43%) in KD in the medulla. The results support the concept that type A γ-aminobutyric acid (GABAa) receptor subtypes are localized differentially throughout the brain. Defining the regional specificity of interactions between benzodiazepines and barbiturates at the GABAa receptor will be important for understanding the mechanisms by which these drugs produce their behavioral effects in vivo.

Original languageEnglish (US)
Pages (from-to)1-10
Number of pages10
JournalPharmacology
Volume49
Issue number1
DOIs
StatePublished - Jan 1 1994

Fingerprint

Flunitrazepam
Pentobarbital
Benzodiazepines
Brain
Aminobutyrates
Barbiturates
Pharmaceutical Preparations

All Science Journal Classification (ASJC) codes

  • Pharmacology

Cite this

Pentobarbital differentially enhances the affinity of [3h]flunitrazepam binding across brain regions. / Carlson, Berit X.; Baghdoyan, Helen.

In: Pharmacology, Vol. 49, No. 1, 01.01.1994, p. 1-10.

Research output: Contribution to journalArticle

@article{018a0616493d4f23be98627285a13a04,
title = "Pentobarbital differentially enhances the affinity of [3h]flunitrazepam binding across brain regions",
abstract = "The present study used saturation binding analyses to test the hypothesis that pentobarbital would differentially increase the affinity of benzodiazepine binding across brain regions. The results showed that there were significant (p < 0.05) regional differences in the dissociation constant (KD) for tritiated fluni- trazepam ([3H]FLU) and that pentobarbital differentially decreased the Kd for [3H]FLU across 6 brain regions. Pentobarbital caused the greatest decrease (−43{\%}) in KD in the medulla. The results support the concept that type A γ-aminobutyric acid (GABAa) receptor subtypes are localized differentially throughout the brain. Defining the regional specificity of interactions between benzodiazepines and barbiturates at the GABAa receptor will be important for understanding the mechanisms by which these drugs produce their behavioral effects in vivo.",
author = "Carlson, {Berit X.} and Helen Baghdoyan",
year = "1994",
month = "1",
day = "1",
doi = "10.1159/000139211",
language = "English (US)",
volume = "49",
pages = "1--10",
journal = "Pharmacology",
issn = "0031-7012",
publisher = "S. Karger AG",
number = "1",

}

TY - JOUR

T1 - Pentobarbital differentially enhances the affinity of [3h]flunitrazepam binding across brain regions

AU - Carlson, Berit X.

AU - Baghdoyan, Helen

PY - 1994/1/1

Y1 - 1994/1/1

N2 - The present study used saturation binding analyses to test the hypothesis that pentobarbital would differentially increase the affinity of benzodiazepine binding across brain regions. The results showed that there were significant (p < 0.05) regional differences in the dissociation constant (KD) for tritiated fluni- trazepam ([3H]FLU) and that pentobarbital differentially decreased the Kd for [3H]FLU across 6 brain regions. Pentobarbital caused the greatest decrease (−43%) in KD in the medulla. The results support the concept that type A γ-aminobutyric acid (GABAa) receptor subtypes are localized differentially throughout the brain. Defining the regional specificity of interactions between benzodiazepines and barbiturates at the GABAa receptor will be important for understanding the mechanisms by which these drugs produce their behavioral effects in vivo.

AB - The present study used saturation binding analyses to test the hypothesis that pentobarbital would differentially increase the affinity of benzodiazepine binding across brain regions. The results showed that there were significant (p < 0.05) regional differences in the dissociation constant (KD) for tritiated fluni- trazepam ([3H]FLU) and that pentobarbital differentially decreased the Kd for [3H]FLU across 6 brain regions. Pentobarbital caused the greatest decrease (−43%) in KD in the medulla. The results support the concept that type A γ-aminobutyric acid (GABAa) receptor subtypes are localized differentially throughout the brain. Defining the regional specificity of interactions between benzodiazepines and barbiturates at the GABAa receptor will be important for understanding the mechanisms by which these drugs produce their behavioral effects in vivo.

UR - http://www.scopus.com/inward/record.url?scp=0028224755&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0028224755&partnerID=8YFLogxK

U2 - 10.1159/000139211

DO - 10.1159/000139211

M3 - Article

VL - 49

SP - 1

EP - 10

JO - Pharmacology

JF - Pharmacology

SN - 0031-7012

IS - 1

ER -