Perivascular blood attenuates noradrenergic but not cholinergic effects on piglet pial arterioles

David W. Busija, Charles Leffler

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

We examined the chronic and acute effects of perivascular blood on cerebrovascular responses to norepinephrine and acetylcholine in 35 piglets. In the chronic experiment, fresh autologous blood (n=15) or cerebrospinal fluid (n=14, control) was placed under the dura mater over the parietal cortex, and the piglets were allowed to recover from anesthesia. One to 4 days later, a closed cranial window was placed over the parietal cortex and baseline pial arteriolar responses and responses to topical application of the neurotransmitters norepinephrine (10-6 and 10-4 M) and acetylcholine (10-4 M) were determined. We also sampled cerebrospinal fluid from under the window during baseline conditions and during application of the neurotransmitters, and we measured the concentrations of prostanoids (6-ketoprostaglandin F1a, thromboxane B2, prostaglandin F1a and prostaglandin E2) via radioimmunoassay. Pial arterioles in the chronic control group (n=13) constricted by 20±2% (mean±SEM) in response to 10-4 M norepinephrine and by 28±2% In response to 10-4 M acetylcholine. In the chronic blood group (n=14), pial arterioles did not constrict significantly in response to 10-4 M norepinephrine but constricted normally (23±4%) in response to 10-4 M acetylcholine. In the acute experiment, six other piglets had blood placed on the brain surface for 30 minutes and then removed; pial arterioles constricted by 21±1% in response to 10-4 M norepinephrine (n=5) and by 28±4% in response to 10-4 M acetylcholine (n=3). Prostaglandin E2 concentrations in the cerebrospinal fluid incre.

Original languageEnglish (US)
Pages (from-to)441-446
Number of pages6
JournalStroke
Volume21
Issue number3
DOIs
StatePublished - Jan 1 1990

Fingerprint

Arterioles
Cholinergic Agents
Acetylcholine
Norepinephrine
Cerebrospinal Fluid
Parietal Lobe
Dinoprostone
Prostaglandins
Neurotransmitter Agents
Dura Mater
Thromboxane B2
Blood Group Antigens
Radioimmunoassay
Anesthesia
Control Groups
Brain

All Science Journal Classification (ASJC) codes

  • Clinical Neurology
  • Cardiology and Cardiovascular Medicine
  • Advanced and Specialized Nursing

Cite this

Perivascular blood attenuates noradrenergic but not cholinergic effects on piglet pial arterioles. / Busija, David W.; Leffler, Charles.

In: Stroke, Vol. 21, No. 3, 01.01.1990, p. 441-446.

Research output: Contribution to journalArticle

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