Permeability of the blood-brain barrier within rat intrastriatal transplants assessed by simultaneous systemic injection of horseradish peroxidase and evans blue dye

Karl J. Bertram, Michael T. Shipley, Matthew Ennis, Paul R. Sanberg, Andrew B. Norman

Research output: Contribution to journalArticle

18 Citations (Scopus)

Abstract

Both horseradish peroxidase (HRP) and Evans blue dye (EBD) have been used previously to characterize the status of the blood-brain barrier (BBB) within brain tissue transplants and host brain tissue. We investigated the possibility that a differential permeability of the vasculature to these two markers can account for discrepancies in the literature concerning the presence of an intact BBB within the grafted tissues. Intravascular injection of both HRP and EBD was used to evaluate the status of the BBB within intracerebral tissue transplants. Simultaneous injection of HRP and EBD in rats with adrenal medulla transplants or C-6 glioma tumors demonstrated a lack of a BBB within these grafts. Both markers produced consistent results within each tissue type, although HRP was generally a more sensitive marker. In contrast to the lack of a BBB in the C-6 gliomas or adrenal medulla transplants, 1-week-old fetal striatal transplants had a BBB essentially intact to both HRP and EBD. Any reported discrepancies in the characterization of the BBB are not likely due to differences between the properties of HRP or EBD. Fetal striatal transplants appear to have an intact BBB at 1 week following transplantation.

Original languageEnglish (US)
Pages (from-to)245-252
Number of pages8
JournalExperimental Neurology
Volume127
Issue number2
DOIs
StatePublished - Jan 1 1994

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Evans Blue
Horseradish Peroxidase
Blood-Brain Barrier
Permeability
Coloring Agents
Transplants
Injections
Corpus Striatum
Adrenal Medulla
Glioma
Brain
Transplantation

All Science Journal Classification (ASJC) codes

  • Neurology
  • Developmental Neuroscience

Cite this

Permeability of the blood-brain barrier within rat intrastriatal transplants assessed by simultaneous systemic injection of horseradish peroxidase and evans blue dye. / Bertram, Karl J.; Shipley, Michael T.; Ennis, Matthew; Sanberg, Paul R.; Norman, Andrew B.

In: Experimental Neurology, Vol. 127, No. 2, 01.01.1994, p. 245-252.

Research output: Contribution to journalArticle

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