Peroxisomal proliferator activated receptor gamma coactivator (PGC-1α) stimulates carnitine palmitoyltransferase I (CPT-Iα) through the first intron

Shulan Song, Yi Zhang, Ke Ma, Loretta Jackson-Hayes, Eduard N. Lavrentyev, George Cook, Marshall Elam, Edwards Park

Research output: Contribution to journalArticle

30 Citations (Scopus)

Abstract

Peroxisomal proliferator activated receptor gamma coactivator-1 (PGC-1α) is a transcriptional coactivator that promotes mitochondrial biogenesis and energy metabolism in brown fat, skeletal muscle and heart. Previous studies demonstrated that PGC-1α is present at low levels in the liver but that the hepatic abundance of PGC-1α is elevated in diabetic and fasted animals. Elevated PGC-1α expression is associated with increased fatty acid oxidation and hepatic glucose production. Carnitine palmitoyltransferase-I (CPT-I) is a rate controlling step in the mitochondrial oxidation of long chain fatty acids. CPT-I transfers the acyl moiety from fatty acyl-CoA to carnitine for the translocation of long chain fatty acids across the mitochondrial membrane. There are two isoforms of CPT-I including a liver isoform CPT-Iα and a muscle isoform CPT-Iβ. Here, we characterized the regulation of CPT-Iα isoform by PGC-1α. PGC-1α stimulates CPT-Iα primarily through multiple sites in the first intron. We found that PGC-1α can induce CPT-Iα gene expression in cardiac myocytes and primary hepatocytes. Our results indicate that PGC-1α elevates the expression of CPT-Iα via a unique mechanism that utilizes elements within the intron.

Original languageEnglish (US)
Pages (from-to)164-173
Number of pages10
JournalBiochimica et Biophysica Acta - Gene Structure and Expression
Volume1679
Issue number2
DOIs
StatePublished - Aug 12 2004

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Carnitine O-Palmitoyltransferase
Introns
Protein Isoforms
Fatty Acids
Liver
Muscle
Oxidation
Acyl Coenzyme A
Brown Adipose Tissue
Carnitine
Mitochondrial Membranes
Organelle Biogenesis
Cardiac Myocytes
Gene expression
Energy Metabolism
Hepatocytes
Animals
Skeletal Muscle
Fats
Membranes

All Science Journal Classification (ASJC) codes

  • Structural Biology
  • Biophysics
  • Biochemistry
  • Genetics

Cite this

Peroxisomal proliferator activated receptor gamma coactivator (PGC-1α) stimulates carnitine palmitoyltransferase I (CPT-Iα) through the first intron. / Song, Shulan; Zhang, Yi; Ma, Ke; Jackson-Hayes, Loretta; Lavrentyev, Eduard N.; Cook, George; Elam, Marshall; Park, Edwards.

In: Biochimica et Biophysica Acta - Gene Structure and Expression, Vol. 1679, No. 2, 12.08.2004, p. 164-173.

Research output: Contribution to journalArticle

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