Pharmacogenetics of cocaine

II. Mesocorticolimbic and striatal dopamine and cocaine receptors in C57BL and DBA mice

V. Gene Erwin, Daniel E. Womer, Andrew D. Campbell, Byron Jones

Research output: Contribution to journalArticle

36 Citations (Scopus)

Abstract

Studies were conducted to determine whether genetic differences in behavioural effects of cocaine in C57BL/6 and DBA/2 mice might be mediated by strain differences in dopamine and serotonin transporters and dopamine D1 and D2 receptors in specific brain regions. Binding characteristics of [3H]CFT, a cocaine analogue, in the presence of either GBR12909, a dopamine uptake blocker or fluoxetine, a serotonin uptake blocker and binding of [3H]-paroxetine, a specific serotonin uptake receptor antagonist, were evaluated. We observed regional differences in [3H]CFT binding parameters in the presence of GBR12909 or fluoxetine, but no strain differences by brain region were observed. There were no differences in [3H]paroxetine binding characteristics between corresponding brain regions from C57BL and DBA/2 mice. The D, antagonist, [3H]SCH23390 and the D2 ligands [3H]sulpiride or [12I]epiderpride were used to determine dopamine receptor characteristics. Regional differences were found in [3H]SCH23390 and [3H]sulpiride, with higher affinities and lower densities in frontal cortex compared to striatum; with no differences in [3H]SCH23390 binding in corresponding tissues from C57BL and DBA/2 brains. There were strain-related differences in [3H]sulpiride and in [3H]epidepride binding in striatal membranes with higher densities in C57BL than in DBA/2. Our findings suggest striatal D2 receptor differences are possibly involved in genetic differences in cocaine-related behaviours.

Original languageEnglish (US)
Pages (from-to)189-196
Number of pages8
JournalPharmacogenetics
Volume3
Issue number4
DOIs
StatePublished - Jan 1 1993

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Corpus Striatum
Inbred DBA Mouse
Pharmacogenetics
Dopamine Receptors
Inbred C57BL Mouse
Cocaine
Sulpiride
Paroxetine
Fluoxetine
Brain
Dopamine D1 Receptors
Serotonin Plasma Membrane Transport Proteins
Serotonin Antagonists
Dopamine Plasma Membrane Transport Proteins
Dopamine Antagonists
Dopamine D2 Receptors
Frontal Lobe
Serotonin
Ligands
Membranes

All Science Journal Classification (ASJC) codes

  • Genetics
  • Pharmacology, Toxicology and Pharmaceutics(all)

Cite this

Pharmacogenetics of cocaine : II. Mesocorticolimbic and striatal dopamine and cocaine receptors in C57BL and DBA mice. / Erwin, V. Gene; Womer, Daniel E.; Campbell, Andrew D.; Jones, Byron.

In: Pharmacogenetics, Vol. 3, No. 4, 01.01.1993, p. 189-196.

Research output: Contribution to journalArticle

Erwin, V. Gene ; Womer, Daniel E. ; Campbell, Andrew D. ; Jones, Byron. / Pharmacogenetics of cocaine : II. Mesocorticolimbic and striatal dopamine and cocaine receptors in C57BL and DBA mice. In: Pharmacogenetics. 1993 ; Vol. 3, No. 4. pp. 189-196.
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