Pharmacological characterization of muscarinic cholinergic receptors in cat pons and cortex

Preliminary Study

Helen Baghdoyan, Berit X. Carlson

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

Muscarinic receptors (mAChRs) in the pontine reticular formation comprise a critical part of the REM sleep-generating system. Although the role of specific mAChR subtypes remains unclear, data from in vivo microinjection studies suggest that in the pons the M2 subtype is important for REM sleep generation. The present study tested the hypothesis that M2 antagonists would show a greater binding potency in feline pons than M1 or M3 antagonists. Competition binding assays showed 4-DAMP to be more potent than pirenzepine or AF- DX 116 in its ability to displace tritiated quinuclidinyl benzi- late, and linear regression analyses indicated that 4-DAMP and pirenzepine each interacted with more than one binding site. These data demonstrate the presence of a mixture of mAChR subtypes in the feline pons, and are consistent with the view that REM sleep is mediated by more than one mAChR subtype.

Original languageEnglish (US)
Pages (from-to)77-85
Number of pages9
JournalPharmacology
Volume48
Issue number2
DOIs
StatePublished - Jan 1 1994
Externally publishedYes

Fingerprint

Pons
REM Sleep
Cholinergic Receptors
Muscarinic Receptors
Pirenzepine
Cats
Felidae
Pharmacology
Quinuclidinyl Benzilate
Aptitude
Microinjections
Linear Models
Binding Sites
Regression Analysis
4-diphenylacetoxy-1,1-dimethylpiperidinium

All Science Journal Classification (ASJC) codes

  • Pharmacology

Cite this

Pharmacological characterization of muscarinic cholinergic receptors in cat pons and cortex : Preliminary Study. / Baghdoyan, Helen; Carlson, Berit X.

In: Pharmacology, Vol. 48, No. 2, 01.01.1994, p. 77-85.

Research output: Contribution to journalArticle

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