Phenotypic and functional characteristic of a newly identified CD8 +Foxp3-CD103+ regulatory T cells

Ya Liu, Qin Lan, Ling Lu, Maogen Chen, Zanxian Xia, Jilin Ma, Julie Wang, Huimin Fan, Yi Shen, Bernhard Ryffel, David Brand, Francisco Quismorio, Zhongmin Liu, David A. Horwitz, Anping Xu, Song Guo Zheng

Research output: Contribution to journalArticle

19 Citations (Scopus)

Abstract

TGF-β and Foxp3 expressions are crucial for the induction and functional activity of CD4+Foxp3+ regulatory T (iTreg) cells. Here, we demonstrate that although TGF-β-primed CD8+ cells display much lower Foxp3 expression, their suppressive capacity is equivalent to that of CD4+ iTreg cells, and both Foxp3- and Foxp3+ CD8+ subsets have suppressive activities in vitro and in vivo. CD8+Foxp3- iTreg cells produce little IFN-γ but almost no IL-2, and display a typical anergic phenotype. Among phenotypic markers expressed in CD8+Foxp3- cells, we identify CD103 expression particularly crucial for the generation and function of this subset. Moreover, IL-10 and TGF-β signals rather than cytotoxicity mediate the suppressive effect of this novel Treg population. Therefore, TGF-β can induce both CD8+Foxp3- and CD8+Foxp3 + iTreg subsets, which may represent the unique immunoregulatory means to treat autoimmune and inflammatory diseases.

Original languageEnglish (US)
Pages (from-to)81-92
Number of pages12
JournalJournal of Molecular Cell Biology
Volume6
Issue number1
DOIs
StatePublished - Feb 1 2014

Fingerprint

Regulatory T-Lymphocytes
Interleukin-10
Autoimmune Diseases
Interleukin-2
Phenotype
Population

All Science Journal Classification (ASJC) codes

  • Molecular Biology
  • Genetics
  • Cell Biology

Cite this

Phenotypic and functional characteristic of a newly identified CD8 +Foxp3-CD103+ regulatory T cells. / Liu, Ya; Lan, Qin; Lu, Ling; Chen, Maogen; Xia, Zanxian; Ma, Jilin; Wang, Julie; Fan, Huimin; Shen, Yi; Ryffel, Bernhard; Brand, David; Quismorio, Francisco; Liu, Zhongmin; Horwitz, David A.; Xu, Anping; Zheng, Song Guo.

In: Journal of Molecular Cell Biology, Vol. 6, No. 1, 01.02.2014, p. 81-92.

Research output: Contribution to journalArticle

Liu, Y, Lan, Q, Lu, L, Chen, M, Xia, Z, Ma, J, Wang, J, Fan, H, Shen, Y, Ryffel, B, Brand, D, Quismorio, F, Liu, Z, Horwitz, DA, Xu, A & Zheng, SG 2014, 'Phenotypic and functional characteristic of a newly identified CD8 +Foxp3-CD103+ regulatory T cells', Journal of Molecular Cell Biology, vol. 6, no. 1, pp. 81-92. https://doi.org/10.1093/jmcb/mjt026
Liu, Ya ; Lan, Qin ; Lu, Ling ; Chen, Maogen ; Xia, Zanxian ; Ma, Jilin ; Wang, Julie ; Fan, Huimin ; Shen, Yi ; Ryffel, Bernhard ; Brand, David ; Quismorio, Francisco ; Liu, Zhongmin ; Horwitz, David A. ; Xu, Anping ; Zheng, Song Guo. / Phenotypic and functional characteristic of a newly identified CD8 +Foxp3-CD103+ regulatory T cells. In: Journal of Molecular Cell Biology. 2014 ; Vol. 6, No. 1. pp. 81-92.
@article{0b00d3db3afd4702829ecad305c0a9a8,
title = "Phenotypic and functional characteristic of a newly identified CD8 +Foxp3-CD103+ regulatory T cells",
abstract = "TGF-β and Foxp3 expressions are crucial for the induction and functional activity of CD4+Foxp3+ regulatory T (iTreg) cells. Here, we demonstrate that although TGF-β-primed CD8+ cells display much lower Foxp3 expression, their suppressive capacity is equivalent to that of CD4+ iTreg cells, and both Foxp3- and Foxp3+ CD8+ subsets have suppressive activities in vitro and in vivo. CD8+Foxp3- iTreg cells produce little IFN-γ but almost no IL-2, and display a typical anergic phenotype. Among phenotypic markers expressed in CD8+Foxp3- cells, we identify CD103 expression particularly crucial for the generation and function of this subset. Moreover, IL-10 and TGF-β signals rather than cytotoxicity mediate the suppressive effect of this novel Treg population. Therefore, TGF-β can induce both CD8+Foxp3- and CD8+Foxp3 + iTreg subsets, which may represent the unique immunoregulatory means to treat autoimmune and inflammatory diseases.",
author = "Ya Liu and Qin Lan and Ling Lu and Maogen Chen and Zanxian Xia and Jilin Ma and Julie Wang and Huimin Fan and Yi Shen and Bernhard Ryffel and David Brand and Francisco Quismorio and Zhongmin Liu and Horwitz, {David A.} and Anping Xu and Zheng, {Song Guo}",
year = "2014",
month = "2",
day = "1",
doi = "10.1093/jmcb/mjt026",
language = "English (US)",
volume = "6",
pages = "81--92",
journal = "Journal of Molecular Cell Biology",
issn = "1674-2788",
publisher = "Oxford University Press",
number = "1",

}

TY - JOUR

T1 - Phenotypic and functional characteristic of a newly identified CD8 +Foxp3-CD103+ regulatory T cells

AU - Liu, Ya

AU - Lan, Qin

AU - Lu, Ling

AU - Chen, Maogen

AU - Xia, Zanxian

AU - Ma, Jilin

AU - Wang, Julie

AU - Fan, Huimin

AU - Shen, Yi

AU - Ryffel, Bernhard

AU - Brand, David

AU - Quismorio, Francisco

AU - Liu, Zhongmin

AU - Horwitz, David A.

AU - Xu, Anping

AU - Zheng, Song Guo

PY - 2014/2/1

Y1 - 2014/2/1

N2 - TGF-β and Foxp3 expressions are crucial for the induction and functional activity of CD4+Foxp3+ regulatory T (iTreg) cells. Here, we demonstrate that although TGF-β-primed CD8+ cells display much lower Foxp3 expression, their suppressive capacity is equivalent to that of CD4+ iTreg cells, and both Foxp3- and Foxp3+ CD8+ subsets have suppressive activities in vitro and in vivo. CD8+Foxp3- iTreg cells produce little IFN-γ but almost no IL-2, and display a typical anergic phenotype. Among phenotypic markers expressed in CD8+Foxp3- cells, we identify CD103 expression particularly crucial for the generation and function of this subset. Moreover, IL-10 and TGF-β signals rather than cytotoxicity mediate the suppressive effect of this novel Treg population. Therefore, TGF-β can induce both CD8+Foxp3- and CD8+Foxp3 + iTreg subsets, which may represent the unique immunoregulatory means to treat autoimmune and inflammatory diseases.

AB - TGF-β and Foxp3 expressions are crucial for the induction and functional activity of CD4+Foxp3+ regulatory T (iTreg) cells. Here, we demonstrate that although TGF-β-primed CD8+ cells display much lower Foxp3 expression, their suppressive capacity is equivalent to that of CD4+ iTreg cells, and both Foxp3- and Foxp3+ CD8+ subsets have suppressive activities in vitro and in vivo. CD8+Foxp3- iTreg cells produce little IFN-γ but almost no IL-2, and display a typical anergic phenotype. Among phenotypic markers expressed in CD8+Foxp3- cells, we identify CD103 expression particularly crucial for the generation and function of this subset. Moreover, IL-10 and TGF-β signals rather than cytotoxicity mediate the suppressive effect of this novel Treg population. Therefore, TGF-β can induce both CD8+Foxp3- and CD8+Foxp3 + iTreg subsets, which may represent the unique immunoregulatory means to treat autoimmune and inflammatory diseases.

UR - http://www.scopus.com/inward/record.url?scp=84896831968&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84896831968&partnerID=8YFLogxK

U2 - 10.1093/jmcb/mjt026

DO - 10.1093/jmcb/mjt026

M3 - Article

VL - 6

SP - 81

EP - 92

JO - Journal of Molecular Cell Biology

JF - Journal of Molecular Cell Biology

SN - 1674-2788

IS - 1

ER -