Phospholipids that contain polyunsaturated fatty acids enhance neuronal cell mechanics and touch sensation

Valeria Vasquez, Michael Krieg, Dean Lockhead, Miriam B. Goodman

Research output: Contribution to journalArticle

45 Citations (Scopus)

Abstract

Mechanoelectrical transduction (MeT) channels embedded in neuronal cell membranes are essential for touch and proprioception. Little is understood about the interplay between native MeT channels and membrane phospholipids, in part because few techniques are available for altering plasma membrane composition invivo. Here, we leverage genetic dissection, chemical complementation, and optogenetics to establish that arachidonic acid (AA), an omega-6 polyunsaturated fatty acid, enhances touch sensation and mechanoelectrical transduction activity while incorporated into membrane phospholipids in C.elegans touch receptor neurons (TRNs). Because dynamic force spectroscopy reveals that AA modulates the mechanical properties of TRN plasma membranes, we propose that this polyunsaturated fatty acid (PUFA) is needed for MeT channel activity. These findings establish that polyunsaturated phospholipids are crucial determinants of both the biochemistry and mechanics of mechanoreceptor neurons and reinforce the idea that sensory mechanotransduction in animals relies on a cellular machine composed of both proteins and membrane lipids.

Original languageEnglish (US)
Pages (from-to)70-80
Number of pages11
JournalCell Reports
Volume6
Issue number1
DOIs
StatePublished - Jan 3 2014

Fingerprint

Touch
Cell membranes
Mechanics
Unsaturated Fatty Acids
Neurons
Phospholipids
Arachidonic Acid
Cell Membrane
Dissection
Biochemistry
Omega-6 Fatty Acids
Optogenetics
Membrane Lipids
Ion Channels
Proprioception
Mechanoreceptors
Animals
Spectroscopy
Proteins
Membranes

All Science Journal Classification (ASJC) codes

  • Biochemistry, Genetics and Molecular Biology(all)

Cite this

Phospholipids that contain polyunsaturated fatty acids enhance neuronal cell mechanics and touch sensation. / Vasquez, Valeria; Krieg, Michael; Lockhead, Dean; Goodman, Miriam B.

In: Cell Reports, Vol. 6, No. 1, 03.01.2014, p. 70-80.

Research output: Contribution to journalArticle

Vasquez, Valeria ; Krieg, Michael ; Lockhead, Dean ; Goodman, Miriam B. / Phospholipids that contain polyunsaturated fatty acids enhance neuronal cell mechanics and touch sensation. In: Cell Reports. 2014 ; Vol. 6, No. 1. pp. 70-80.
@article{b09fa684052245e19216b92c06bc6173,
title = "Phospholipids that contain polyunsaturated fatty acids enhance neuronal cell mechanics and touch sensation",
abstract = "Mechanoelectrical transduction (MeT) channels embedded in neuronal cell membranes are essential for touch and proprioception. Little is understood about the interplay between native MeT channels and membrane phospholipids, in part because few techniques are available for altering plasma membrane composition invivo. Here, we leverage genetic dissection, chemical complementation, and optogenetics to establish that arachidonic acid (AA), an omega-6 polyunsaturated fatty acid, enhances touch sensation and mechanoelectrical transduction activity while incorporated into membrane phospholipids in C.elegans touch receptor neurons (TRNs). Because dynamic force spectroscopy reveals that AA modulates the mechanical properties of TRN plasma membranes, we propose that this polyunsaturated fatty acid (PUFA) is needed for MeT channel activity. These findings establish that polyunsaturated phospholipids are crucial determinants of both the biochemistry and mechanics of mechanoreceptor neurons and reinforce the idea that sensory mechanotransduction in animals relies on a cellular machine composed of both proteins and membrane lipids.",
author = "Valeria Vasquez and Michael Krieg and Dean Lockhead and Goodman, {Miriam B.}",
year = "2014",
month = "1",
day = "3",
doi = "10.1016/j.celrep.2013.12.012",
language = "English (US)",
volume = "6",
pages = "70--80",
journal = "Cell Reports",
issn = "2211-1247",
publisher = "Cell Press",
number = "1",

}

TY - JOUR

T1 - Phospholipids that contain polyunsaturated fatty acids enhance neuronal cell mechanics and touch sensation

AU - Vasquez, Valeria

AU - Krieg, Michael

AU - Lockhead, Dean

AU - Goodman, Miriam B.

PY - 2014/1/3

Y1 - 2014/1/3

N2 - Mechanoelectrical transduction (MeT) channels embedded in neuronal cell membranes are essential for touch and proprioception. Little is understood about the interplay between native MeT channels and membrane phospholipids, in part because few techniques are available for altering plasma membrane composition invivo. Here, we leverage genetic dissection, chemical complementation, and optogenetics to establish that arachidonic acid (AA), an omega-6 polyunsaturated fatty acid, enhances touch sensation and mechanoelectrical transduction activity while incorporated into membrane phospholipids in C.elegans touch receptor neurons (TRNs). Because dynamic force spectroscopy reveals that AA modulates the mechanical properties of TRN plasma membranes, we propose that this polyunsaturated fatty acid (PUFA) is needed for MeT channel activity. These findings establish that polyunsaturated phospholipids are crucial determinants of both the biochemistry and mechanics of mechanoreceptor neurons and reinforce the idea that sensory mechanotransduction in animals relies on a cellular machine composed of both proteins and membrane lipids.

AB - Mechanoelectrical transduction (MeT) channels embedded in neuronal cell membranes are essential for touch and proprioception. Little is understood about the interplay between native MeT channels and membrane phospholipids, in part because few techniques are available for altering plasma membrane composition invivo. Here, we leverage genetic dissection, chemical complementation, and optogenetics to establish that arachidonic acid (AA), an omega-6 polyunsaturated fatty acid, enhances touch sensation and mechanoelectrical transduction activity while incorporated into membrane phospholipids in C.elegans touch receptor neurons (TRNs). Because dynamic force spectroscopy reveals that AA modulates the mechanical properties of TRN plasma membranes, we propose that this polyunsaturated fatty acid (PUFA) is needed for MeT channel activity. These findings establish that polyunsaturated phospholipids are crucial determinants of both the biochemistry and mechanics of mechanoreceptor neurons and reinforce the idea that sensory mechanotransduction in animals relies on a cellular machine composed of both proteins and membrane lipids.

UR - http://www.scopus.com/inward/record.url?scp=84892511176&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84892511176&partnerID=8YFLogxK

U2 - 10.1016/j.celrep.2013.12.012

DO - 10.1016/j.celrep.2013.12.012

M3 - Article

VL - 6

SP - 70

EP - 80

JO - Cell Reports

JF - Cell Reports

SN - 2211-1247

IS - 1

ER -