Phosphoproteome discovery in human biological fluids

Research output: Contribution to journalReview article

5 Citations (Scopus)

Abstract

Phosphorylation plays a critical role in regulating protein function and thus influences a vast spectrum of cellular processes. With the advent of modern bioanalytical technologies, examination of protein phosphorylation on a global scale has become one of the major research areas. Phosphoproteins are found in biological fluids and interrogation of the phosphoproteome in biological fluids presents an exciting opportunity for discoveries that hold great potential for novel mechanistic insights into protein function in health and disease, and for translation to improved diagnostic and therapeutic approaches for the clinical setting. This review focuses on phosphoproteome discovery in selected human biological fluids: serum/plasma, urine, cerebrospinal fluid, saliva, and bronchoalveolar lavage fluid. Bioanalytical workflows pertinent to phosphoproteomics of biological fluids are discussed with emphasis on mass spectrometry-based approaches, and summaries of studies on phosphoproteome discovery in major fluids are presented.

Original languageEnglish (US)
Article number37
JournalProteomes
Volume4
Issue number4
DOIs
StatePublished - Dec 1 2016

Fingerprint

Fluids
Phosphorylation
Proteins
Workflow
Phosphoproteins
Bronchoalveolar Lavage Fluid
Saliva
Cerebrospinal Fluid
Mass Spectrometry
Urine
Technology
Cerebrospinal fluid
Health
Serum
Research
Mass spectrometry
Plasmas
Therapeutics

All Science Journal Classification (ASJC) codes

  • Structural Biology
  • Biochemistry
  • Molecular Biology
  • Clinical Biochemistry

Cite this

Phosphoproteome discovery in human biological fluids. / Giorgianni, Francesco; Beranova, Sarka.

In: Proteomes, Vol. 4, No. 4, 37, 01.12.2016.

Research output: Contribution to journalReview article

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