Physical properties and in vitro evaluation of collagen-chitosan-calcium phosphate microparticle-based scaffolds for bone tissue regeneration

Monica V. Zugravu, Richard Smith, Benjamin T. Reves, Jessica A. Jennings, Jared O. Cooper, Warren O. Haggard, Joel D. Bumgardner

Research output: Contribution to journalArticle

19 Citations (Scopus)

Abstract

Due to limitations of bone autografts and allografts, synthetic bone grafts using osteoconductive biomaterials have been designed. In this study, collagen-chitosan-calcium phosphate microparticle-based scaffolds fused with glycolic acid were compared to their counterparts without collagen in terms of degradation, cytocompatibility, porosity, and Young's modulus. It was found that 26-30% collagen was incorporated and that hydroxyapatite was present. Moreover, there were no differences between control and collagen scaffolds in degradation, cytocompatibility, porosity, and Young's modulus. In general, scaffolds exhibited 23% porosity, 0.6-1.2 MPa Young's modulus, 23% degradation over 4 weeks, and supported a four to seven fold increase in osteoblast cell number over 7 days in culture. Collagen can be incorporated into these bone graft substitute scaffolds, which show an improved degradation profile.

Original languageEnglish (US)
Pages (from-to)566-579
Number of pages14
JournalJournal of Biomaterials Applications
Volume28
Issue number4
DOIs
StatePublished - Nov 1 2013

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Tissue regeneration
Calcium phosphate
Chitosan
Scaffolds (biology)
Collagen
Scaffolds
Bone
Physical properties
Degradation
glycolic acid
Porosity
Elastic moduli
Grafts
Osteoblasts
Autografts
Biocompatible Materials
Durapatite
Hydroxyapatite
Cell culture
Biomaterials

All Science Journal Classification (ASJC) codes

  • Biomaterials
  • Biomedical Engineering

Cite this

Physical properties and in vitro evaluation of collagen-chitosan-calcium phosphate microparticle-based scaffolds for bone tissue regeneration. / Zugravu, Monica V.; Smith, Richard; Reves, Benjamin T.; Jennings, Jessica A.; Cooper, Jared O.; Haggard, Warren O.; Bumgardner, Joel D.

In: Journal of Biomaterials Applications, Vol. 28, No. 4, 01.11.2013, p. 566-579.

Research output: Contribution to journalArticle

Zugravu, Monica V. ; Smith, Richard ; Reves, Benjamin T. ; Jennings, Jessica A. ; Cooper, Jared O. ; Haggard, Warren O. ; Bumgardner, Joel D. / Physical properties and in vitro evaluation of collagen-chitosan-calcium phosphate microparticle-based scaffolds for bone tissue regeneration. In: Journal of Biomaterials Applications. 2013 ; Vol. 28, No. 4. pp. 566-579.
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