Physicochemical and Immunological Studies of the Renatured α1(II) Chains and Isolated Cyanogen Bromide Peptides of Type II Collagen

Kuniaki Terato, Michael A. Cremer, Karen Hasty, Andrew Kang, David L. Hasty, Alexander S. Townes

Research output: Contribution to journalArticle

20 Citations (Scopus)

Abstract

The α1(II) and cyanogen bromide (CB)1-generated peptides of chick type II collagen were isolated, purified, renatured and examined for their physicochemical and immunological properties. The α1(II) chains and peptides CB-6 through CB-12 (3,000 to 40,000 daltons) formed renatured thermostable products as determined by measurements of reduced viscosity, optical rotation and Stokes radius. Moreover, renatured α1 (II) chains and CB-10 were observed to form segment-long-spacing (SLS) crystallites under appropriate conditions. When examined for immunoreactivity with defined rat polyclonal and mouse monoclonal antibodies to chick type II collagen, conformation-dependent epitopes were detected on renatured a1(II) chains and renatured peptides, CB-8, CB-10 and CB-11. Conformation-independent epitopes were also detected on all CB-peptides in their denatured form. These studies demonstrate that the α1(II) chains and CB-peptides of chick type II collagen can be efficiently renatured and that the renatured products retain some conformation-dependent epitopes present on the naive molecule.

Original languageEnglish (US)
Pages (from-to)469-480
Number of pages12
JournalTopics in Catalysis
Volume5
Issue number6
DOIs
StatePublished - Jan 1 1985

Fingerprint

Cyanogen Bromide
Collagen Type II
Collagen
Peptides
Epitopes
Conformations
Optical rotation
Monoclonal antibodies
Crystallites
Rats
Optical Rotation
Viscosity
Molecules
Monoclonal Antibodies

All Science Journal Classification (ASJC) codes

  • Catalysis
  • Chemistry(all)
  • Rheumatology

Cite this

Physicochemical and Immunological Studies of the Renatured α1(II) Chains and Isolated Cyanogen Bromide Peptides of Type II Collagen. / Terato, Kuniaki; Cremer, Michael A.; Hasty, Karen; Kang, Andrew; Hasty, David L.; Townes, Alexander S.

In: Topics in Catalysis, Vol. 5, No. 6, 01.01.1985, p. 469-480.

Research output: Contribution to journalArticle

Terato, Kuniaki ; Cremer, Michael A. ; Hasty, Karen ; Kang, Andrew ; Hasty, David L. ; Townes, Alexander S. / Physicochemical and Immunological Studies of the Renatured α1(II) Chains and Isolated Cyanogen Bromide Peptides of Type II Collagen. In: Topics in Catalysis. 1985 ; Vol. 5, No. 6. pp. 469-480.
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