Phytoestrogen Genistein induces osteoblastic differentiation of mouse bone marrow-derived mesenchymal stem cells through p38 MAPK

Qing Chuan Liao, Zhousheng Xiao, Yan Fang Qin, Ting Liu, Yan Zhao, Hong Hao Zhou

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Abstract

Aim: To investigate the role of p38 mitogen-activated protein kinases (MAPKs) in genistein-induced osteoblastic differentiation of mouse bone marrow-derived mesenchymal stem cells (BMSCs). Methods: Mouse BMSCs cultured in phenol red-free α-MEM containing 10% V/V FBS, were added β-glycerophosphate and ascorbic acid for inducing osteoblastic differentiation, and treated with 0.01 ∼ 1 μmol · L-1 genistein and/or SB203580 and PD98059. The temporal sequence of osteoblastic differentiation in BMSCs cultures was assayed by measuring alkaline phosphatase activity (ALP) and calcium deposition. The MAPK phosphorylation level was detected by Westernblot. Results: Genistein (0-01 ∼ 1 μmol · L-1) showed a dose-dependent effect on osteoblastic differentiation as evidenced by increased alkaline phosphatase (ALP) activity and calcium deposition in mouse BMSCs cultures. Genistein (1 μmol · L -1)-induced osteoblastic differentiation of BMSCs was diminished by p38 MAPK inhibitor but not the p44/42 MAPK inhibitor. The effects of Genistein were associated with rapid and sustained activation of p38 MAPK in the BMSCs cultures, which was blocked by SB203580 (1 μmol · L-1). In contrast, Genistein treatment resulted in inactivation of p42/44MAPK, which was further attenuated by PD98059 (25 μmol · L-1). Conclusion: p38 MAPK plays an important role in genistein-induced osteoblastic differentiation of mouse BMSCs cultures.

Original languageEnglish (US)
Pages (from-to)683-687
Number of pages5
JournalChinese Pharmacological Bulletin
Volume22
Issue number6
StatePublished - Jan 1 2006

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Phytoestrogens
Genistein
p38 Mitogen-Activated Protein Kinases
Mesenchymal Stromal Cells
Bone Marrow
Cell Culture Techniques
Protein Kinase Inhibitors
Mitogen-Activated Protein Kinases
Alkaline Phosphatase
Phenolsulfonphthalein
Calcium
Glycerophosphates
Ascorbic Acid
Phosphorylation

All Science Journal Classification (ASJC) codes

  • Pharmacology

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Phytoestrogen Genistein induces osteoblastic differentiation of mouse bone marrow-derived mesenchymal stem cells through p38 MAPK. / Liao, Qing Chuan; Xiao, Zhousheng; Qin, Yan Fang; Liu, Ting; Zhao, Yan; Zhou, Hong Hao.

In: Chinese Pharmacological Bulletin, Vol. 22, No. 6, 01.01.2006, p. 683-687.

Research output: Contribution to journalArticle

Liao, Qing Chuan ; Xiao, Zhousheng ; Qin, Yan Fang ; Liu, Ting ; Zhao, Yan ; Zhou, Hong Hao. / Phytoestrogen Genistein induces osteoblastic differentiation of mouse bone marrow-derived mesenchymal stem cells through p38 MAPK. In: Chinese Pharmacological Bulletin. 2006 ; Vol. 22, No. 6. pp. 683-687.
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abstract = "Aim: To investigate the role of p38 mitogen-activated protein kinases (MAPKs) in genistein-induced osteoblastic differentiation of mouse bone marrow-derived mesenchymal stem cells (BMSCs). Methods: Mouse BMSCs cultured in phenol red-free α-MEM containing 10{\%} V/V FBS, were added β-glycerophosphate and ascorbic acid for inducing osteoblastic differentiation, and treated with 0.01 ∼ 1 μmol · L-1 genistein and/or SB203580 and PD98059. The temporal sequence of osteoblastic differentiation in BMSCs cultures was assayed by measuring alkaline phosphatase activity (ALP) and calcium deposition. The MAPK phosphorylation level was detected by Westernblot. Results: Genistein (0-01 ∼ 1 μmol · L-1) showed a dose-dependent effect on osteoblastic differentiation as evidenced by increased alkaline phosphatase (ALP) activity and calcium deposition in mouse BMSCs cultures. Genistein (1 μmol · L -1)-induced osteoblastic differentiation of BMSCs was diminished by p38 MAPK inhibitor but not the p44/42 MAPK inhibitor. The effects of Genistein were associated with rapid and sustained activation of p38 MAPK in the BMSCs cultures, which was blocked by SB203580 (1 μmol · L-1). In contrast, Genistein treatment resulted in inactivation of p42/44MAPK, which was further attenuated by PD98059 (25 μmol · L-1). Conclusion: p38 MAPK plays an important role in genistein-induced osteoblastic differentiation of mouse BMSCs cultures.",
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N2 - Aim: To investigate the role of p38 mitogen-activated protein kinases (MAPKs) in genistein-induced osteoblastic differentiation of mouse bone marrow-derived mesenchymal stem cells (BMSCs). Methods: Mouse BMSCs cultured in phenol red-free α-MEM containing 10% V/V FBS, were added β-glycerophosphate and ascorbic acid for inducing osteoblastic differentiation, and treated with 0.01 ∼ 1 μmol · L-1 genistein and/or SB203580 and PD98059. The temporal sequence of osteoblastic differentiation in BMSCs cultures was assayed by measuring alkaline phosphatase activity (ALP) and calcium deposition. The MAPK phosphorylation level was detected by Westernblot. Results: Genistein (0-01 ∼ 1 μmol · L-1) showed a dose-dependent effect on osteoblastic differentiation as evidenced by increased alkaline phosphatase (ALP) activity and calcium deposition in mouse BMSCs cultures. Genistein (1 μmol · L -1)-induced osteoblastic differentiation of BMSCs was diminished by p38 MAPK inhibitor but not the p44/42 MAPK inhibitor. The effects of Genistein were associated with rapid and sustained activation of p38 MAPK in the BMSCs cultures, which was blocked by SB203580 (1 μmol · L-1). In contrast, Genistein treatment resulted in inactivation of p42/44MAPK, which was further attenuated by PD98059 (25 μmol · L-1). Conclusion: p38 MAPK plays an important role in genistein-induced osteoblastic differentiation of mouse BMSCs cultures.

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