Pilocytic astrocytoma of the optic pathway: A tumour deriving from radial glia cells with a specific gene signature

Aurlie Tchoghandjian, Carla Fernandez, Carole Colin, Ikbale El Ayachi, Brigitte Voutsinos-Porche, Frdric Fina, Didier Scavarda, Marie Dominique Piercecchi-Marti, Dominique Intagliata, L'Houcine Ouafik, Caroline Fraslon-Vanhulle, Dominique Figarella-Branger

Research output: Contribution to journalArticle

37 Citations (Scopus)

Abstract

Pilocytic astrocytomas are WHO grade I gliomas that occur predominantly in childhood. They share features of both astroglial and oligodendroglial lineages. These tumours affect preferentially the cerebellum (benign clinical course) and the optic pathway, especially the hypothalamochiasmatic region (poor prognosis). Understanding the molecular basis responsible for the aggressive behaviour of hypothalamochiasmatic pilocytic astrocytomas is a prerequisite to setting up new molecular targeted therapies. We used the microarray technique to compare the transcriptional profiles of five hypothalamochiasmatic and six cerebellar pilocytic astrocytomas. Validation of the microarray results and comparison of the tumours with normal developing tissue was done by quantitative real-time PCR and immunohistochemistry. Results demonstrate that cerebellar and hypothalamochiasmatic pilocytic astrocytomas are two genetically distinct and topography-dependent entities. Numerous genes upregulated in hypothalamochiasmatic pilocytic astrocytomas also increased in the developing chiasm, suggesting that developmental genes mirror the cell of origin whereas migrative, adhesive and proliferative genes reflect infiltrative properties of these tumours. Of particular interest, NOTCH2, a gene expressed in radial glia and involved in gliomagenesis, was upregulated in hypothalamochiasmatic pilocytic astrocytomas. In order to find progenitor cells that could give rise to hypothalamo-chiasmatic pilocytic astrocytomas, we performed a morphological study of the hypothalamochiasmatic region and identified, in the floor of the third ventricle, a unique population of vimentin- and glial fibrillary acidic protein-positive cells highly suggestive of radial glia cells. Therefore, pilocytic astrocytomas of the hypothalamochiasmatic region should be considered as a distinct entity which probably originates from a unique population of cells with radial glia phenotype.

Original languageEnglish (US)
Pages (from-to)1523-1535
Number of pages13
JournalBrain
Volume132
Issue number6
DOIs
StatePublished - Jun 1 2009

Fingerprint

Astrocytoma
Neuroglia
Genes
Neoplasms
Molecular Targeted Therapy
Developmental Genes
Third Ventricle
Glial Fibrillary Acidic Protein
Vimentin
Glioma
Adhesives
Cerebellum
Population
Real-Time Polymerase Chain Reaction
Stem Cells
Immunohistochemistry
Phenotype

All Science Journal Classification (ASJC) codes

  • Clinical Neurology

Cite this

Tchoghandjian, A., Fernandez, C., Colin, C., El Ayachi, I., Voutsinos-Porche, B., Fina, F., ... Figarella-Branger, D. (2009). Pilocytic astrocytoma of the optic pathway: A tumour deriving from radial glia cells with a specific gene signature. Brain, 132(6), 1523-1535. https://doi.org/10.1093/brain/awp048

Pilocytic astrocytoma of the optic pathway : A tumour deriving from radial glia cells with a specific gene signature. / Tchoghandjian, Aurlie; Fernandez, Carla; Colin, Carole; El Ayachi, Ikbale; Voutsinos-Porche, Brigitte; Fina, Frdric; Scavarda, Didier; Piercecchi-Marti, Marie Dominique; Intagliata, Dominique; Ouafik, L'Houcine; Fraslon-Vanhulle, Caroline; Figarella-Branger, Dominique.

In: Brain, Vol. 132, No. 6, 01.06.2009, p. 1523-1535.

Research output: Contribution to journalArticle

Tchoghandjian, A, Fernandez, C, Colin, C, El Ayachi, I, Voutsinos-Porche, B, Fina, F, Scavarda, D, Piercecchi-Marti, MD, Intagliata, D, Ouafik, LH, Fraslon-Vanhulle, C & Figarella-Branger, D 2009, 'Pilocytic astrocytoma of the optic pathway: A tumour deriving from radial glia cells with a specific gene signature', Brain, vol. 132, no. 6, pp. 1523-1535. https://doi.org/10.1093/brain/awp048
Tchoghandjian, Aurlie ; Fernandez, Carla ; Colin, Carole ; El Ayachi, Ikbale ; Voutsinos-Porche, Brigitte ; Fina, Frdric ; Scavarda, Didier ; Piercecchi-Marti, Marie Dominique ; Intagliata, Dominique ; Ouafik, L'Houcine ; Fraslon-Vanhulle, Caroline ; Figarella-Branger, Dominique. / Pilocytic astrocytoma of the optic pathway : A tumour deriving from radial glia cells with a specific gene signature. In: Brain. 2009 ; Vol. 132, No. 6. pp. 1523-1535.
@article{6432c301cb3646da9d795bb9a48cb911,
title = "Pilocytic astrocytoma of the optic pathway: A tumour deriving from radial glia cells with a specific gene signature",
abstract = "Pilocytic astrocytomas are WHO grade I gliomas that occur predominantly in childhood. They share features of both astroglial and oligodendroglial lineages. These tumours affect preferentially the cerebellum (benign clinical course) and the optic pathway, especially the hypothalamochiasmatic region (poor prognosis). Understanding the molecular basis responsible for the aggressive behaviour of hypothalamochiasmatic pilocytic astrocytomas is a prerequisite to setting up new molecular targeted therapies. We used the microarray technique to compare the transcriptional profiles of five hypothalamochiasmatic and six cerebellar pilocytic astrocytomas. Validation of the microarray results and comparison of the tumours with normal developing tissue was done by quantitative real-time PCR and immunohistochemistry. Results demonstrate that cerebellar and hypothalamochiasmatic pilocytic astrocytomas are two genetically distinct and topography-dependent entities. Numerous genes upregulated in hypothalamochiasmatic pilocytic astrocytomas also increased in the developing chiasm, suggesting that developmental genes mirror the cell of origin whereas migrative, adhesive and proliferative genes reflect infiltrative properties of these tumours. Of particular interest, NOTCH2, a gene expressed in radial glia and involved in gliomagenesis, was upregulated in hypothalamochiasmatic pilocytic astrocytomas. In order to find progenitor cells that could give rise to hypothalamo-chiasmatic pilocytic astrocytomas, we performed a morphological study of the hypothalamochiasmatic region and identified, in the floor of the third ventricle, a unique population of vimentin- and glial fibrillary acidic protein-positive cells highly suggestive of radial glia cells. Therefore, pilocytic astrocytomas of the hypothalamochiasmatic region should be considered as a distinct entity which probably originates from a unique population of cells with radial glia phenotype.",
author = "Aurlie Tchoghandjian and Carla Fernandez and Carole Colin and {El Ayachi}, Ikbale and Brigitte Voutsinos-Porche and Frdric Fina and Didier Scavarda and Piercecchi-Marti, {Marie Dominique} and Dominique Intagliata and L'Houcine Ouafik and Caroline Fraslon-Vanhulle and Dominique Figarella-Branger",
year = "2009",
month = "6",
day = "1",
doi = "10.1093/brain/awp048",
language = "English (US)",
volume = "132",
pages = "1523--1535",
journal = "Brain",
issn = "0006-8950",
publisher = "Oxford University Press",
number = "6",

}

TY - JOUR

T1 - Pilocytic astrocytoma of the optic pathway

T2 - A tumour deriving from radial glia cells with a specific gene signature

AU - Tchoghandjian, Aurlie

AU - Fernandez, Carla

AU - Colin, Carole

AU - El Ayachi, Ikbale

AU - Voutsinos-Porche, Brigitte

AU - Fina, Frdric

AU - Scavarda, Didier

AU - Piercecchi-Marti, Marie Dominique

AU - Intagliata, Dominique

AU - Ouafik, L'Houcine

AU - Fraslon-Vanhulle, Caroline

AU - Figarella-Branger, Dominique

PY - 2009/6/1

Y1 - 2009/6/1

N2 - Pilocytic astrocytomas are WHO grade I gliomas that occur predominantly in childhood. They share features of both astroglial and oligodendroglial lineages. These tumours affect preferentially the cerebellum (benign clinical course) and the optic pathway, especially the hypothalamochiasmatic region (poor prognosis). Understanding the molecular basis responsible for the aggressive behaviour of hypothalamochiasmatic pilocytic astrocytomas is a prerequisite to setting up new molecular targeted therapies. We used the microarray technique to compare the transcriptional profiles of five hypothalamochiasmatic and six cerebellar pilocytic astrocytomas. Validation of the microarray results and comparison of the tumours with normal developing tissue was done by quantitative real-time PCR and immunohistochemistry. Results demonstrate that cerebellar and hypothalamochiasmatic pilocytic astrocytomas are two genetically distinct and topography-dependent entities. Numerous genes upregulated in hypothalamochiasmatic pilocytic astrocytomas also increased in the developing chiasm, suggesting that developmental genes mirror the cell of origin whereas migrative, adhesive and proliferative genes reflect infiltrative properties of these tumours. Of particular interest, NOTCH2, a gene expressed in radial glia and involved in gliomagenesis, was upregulated in hypothalamochiasmatic pilocytic astrocytomas. In order to find progenitor cells that could give rise to hypothalamo-chiasmatic pilocytic astrocytomas, we performed a morphological study of the hypothalamochiasmatic region and identified, in the floor of the third ventricle, a unique population of vimentin- and glial fibrillary acidic protein-positive cells highly suggestive of radial glia cells. Therefore, pilocytic astrocytomas of the hypothalamochiasmatic region should be considered as a distinct entity which probably originates from a unique population of cells with radial glia phenotype.

AB - Pilocytic astrocytomas are WHO grade I gliomas that occur predominantly in childhood. They share features of both astroglial and oligodendroglial lineages. These tumours affect preferentially the cerebellum (benign clinical course) and the optic pathway, especially the hypothalamochiasmatic region (poor prognosis). Understanding the molecular basis responsible for the aggressive behaviour of hypothalamochiasmatic pilocytic astrocytomas is a prerequisite to setting up new molecular targeted therapies. We used the microarray technique to compare the transcriptional profiles of five hypothalamochiasmatic and six cerebellar pilocytic astrocytomas. Validation of the microarray results and comparison of the tumours with normal developing tissue was done by quantitative real-time PCR and immunohistochemistry. Results demonstrate that cerebellar and hypothalamochiasmatic pilocytic astrocytomas are two genetically distinct and topography-dependent entities. Numerous genes upregulated in hypothalamochiasmatic pilocytic astrocytomas also increased in the developing chiasm, suggesting that developmental genes mirror the cell of origin whereas migrative, adhesive and proliferative genes reflect infiltrative properties of these tumours. Of particular interest, NOTCH2, a gene expressed in radial glia and involved in gliomagenesis, was upregulated in hypothalamochiasmatic pilocytic astrocytomas. In order to find progenitor cells that could give rise to hypothalamo-chiasmatic pilocytic astrocytomas, we performed a morphological study of the hypothalamochiasmatic region and identified, in the floor of the third ventricle, a unique population of vimentin- and glial fibrillary acidic protein-positive cells highly suggestive of radial glia cells. Therefore, pilocytic astrocytomas of the hypothalamochiasmatic region should be considered as a distinct entity which probably originates from a unique population of cells with radial glia phenotype.

UR - http://www.scopus.com/inward/record.url?scp=67649400757&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=67649400757&partnerID=8YFLogxK

U2 - 10.1093/brain/awp048

DO - 10.1093/brain/awp048

M3 - Article

C2 - 19336457

AN - SCOPUS:67649400757

VL - 132

SP - 1523

EP - 1535

JO - Brain

JF - Brain

SN - 0006-8950

IS - 6

ER -