Pituitary Adenylate Cyclase-Activating Polypeptide Dilates Cerebral Arterioles of Newborn Pigs

William M. Armstead, Charles Leffler, Shiwei Tong, Elena Parfenova, Masaaki Shibata, Samuel Zuckerman

Research output: Contribution to journalArticle

19 Citations (Scopus)

Abstract

The actions of the 38- and 27-amino acid forms of synthetic pituitary adenylate cyclase-activating polypeptide (PACAP-38 and PACAP-27) on cerebral arterioles were tested in anesthetized newborn pigs equipped with closed cranial windows. The diameter changes of pial arterioles to topical PACAP were measured and cortical periarach-noid cerebrospinal fluid samples were collected for measurement of cAMP. The 38- and 27-amino acid forms of PACAP produced similar dose-dependent vasodilations. The increases of pial arteriolar diameter produced by PACAP-38 were 6 ± 1%, 15 ± 2%, 23 ± 3%, and 38 ± 3%, and those produced by PACAP-27 were 6 ± 1%, 15 ± 2%, 27 ± 5%, and 38 ± 8% at 10−9, 10−8, 10−7, and 10−6<M, respectively. Arteriolar diameter began to increase 1-2 min and reached a maximum 6-8 min after topical application of PACAP. Vasoactive concentrations of PACAP-38 and PACAP-27 increased cerebrospinal fluid cAMP levels dose dependently. Thus, PACAP-38 and PACAP-27 appear to stimulate cerebral adenylate cyclase and are potent dilators of newborn pigs pial arterioles.

Original languageEnglish (US)
Pages (from-to)343-347
Number of pages5
JournalProceedings of the Society for Experimental Biology and Medicine
Volume203
Issue number3
DOIs
StatePublished - Jan 1 1993

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Pituitary Adenylate Cyclase-Activating Polypeptide
Arterioles
Swine
Cerebrospinal fluid
Cerebrospinal Fluid
Amino Acids
Adenylyl Cyclases
Vasodilation

All Science Journal Classification (ASJC) codes

  • Biochemistry, Genetics and Molecular Biology(all)

Cite this

Pituitary Adenylate Cyclase-Activating Polypeptide Dilates Cerebral Arterioles of Newborn Pigs. / Armstead, William M.; Leffler, Charles; Tong, Shiwei; Parfenova, Elena; Shibata, Masaaki; Zuckerman, Samuel.

In: Proceedings of the Society for Experimental Biology and Medicine, Vol. 203, No. 3, 01.01.1993, p. 343-347.

Research output: Contribution to journalArticle

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