Plasticity of stem cells derived from adult periodontal ligament

C. Y.Charles Huang, Daniel Pelaez, Juan Dominguez Bendala, Franklin Garcia-Godoy, Herman S. Cheung

Research output: Contribution to journalArticle

81 Citations (Scopus)

Abstract

Background: The neural crest contains pluripotent cells that can give rise to neurons and glial cells of the peripheral nervous system, endocrine cells, connective tissue cells, muscle cells and pigment cells during embryonic development. Stem cells derived from the neural crest may still reside in neural crest derivatives including the periodontal ligament (PDL). However, the pluripotency of PDL-derived stem cells has not been investigated. Aim: To identify subpopulations of stem cells from the adult PDL and study their pluripotency. Human PDLs were harvested from impacted wisdom teeth (patients aged 19-22 years). Results: This study demonstrated that subpopulations of PDL cells expressed embryonic stem cell markers (Oct4, Sox2, Nanog and Klf4) and a subset of neural crest markers (Nestin, Slug, p75 and Sox10). Such PDL cell subpopulations exhibited the potential to differentiate into neurogenic, cardiomyogenic, chondrogenic and osteogenic lineages. Furthermore, preliminary evidence suggesting insulin production of PDL cells might be indicative of the generation of cells of the endodermal lineage. Conclusion: These findings suggest that the PDL may contain pluripotent stem cells that originate from the neural crest. Our observations open the door to prospective autologous therapeutic applications for a variety of conditions.

Original languageEnglish (US)
Pages (from-to)809-821
Number of pages13
JournalRegenerative Medicine
Volume4
Issue number6
DOIs
StatePublished - Nov 1 2009

Fingerprint

Periodontal Ligament
Adult Stem Cells
Ligaments
Stem cells
Plasticity
Neural Crest
Cells
Impacted Tooth
Connective Tissue Cells
Nestin
Pluripotent Stem Cells
Gastropoda
Third Molar
Endocrine Cells
Neural Stem Cells
Insulin
Peripheral Nervous System
Neurology
Cell Lineage
Embryonic Stem Cells

All Science Journal Classification (ASJC) codes

  • Biomedical Engineering
  • Embryology

Cite this

Plasticity of stem cells derived from adult periodontal ligament. / Huang, C. Y.Charles; Pelaez, Daniel; Bendala, Juan Dominguez; Garcia-Godoy, Franklin; Cheung, Herman S.

In: Regenerative Medicine, Vol. 4, No. 6, 01.11.2009, p. 809-821.

Research output: Contribution to journalArticle

Huang, C. Y.Charles ; Pelaez, Daniel ; Bendala, Juan Dominguez ; Garcia-Godoy, Franklin ; Cheung, Herman S. / Plasticity of stem cells derived from adult periodontal ligament. In: Regenerative Medicine. 2009 ; Vol. 4, No. 6. pp. 809-821.
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