Platelet activating factor induces expression of early response genes c-fos and TIS-1 in human epidermoid carcinoma A-431 cells

Yamini B. Tripathi, Jagannadha C. Kandala, Ramareddy Guntaka, Robert W. Lim, Shivendra D. Shukla

Research output: Contribution to journalArticle

26 Citations (Scopus)

Abstract

The effect of platelet activating factor (PAF) on the induction of early response genes was investigated in A-431 cells (human epidermal carcinoma cells). PAF induced a transient expression of c-fos and TIS-1 mRNA in a time- and dose-dependent manner. As low as 10-10 M PAF caused detectable expression of these genes with a maximum observed at 10-7 M. In the presence of cycloheximide, increases in the gene expression were noticeable at 20 min and peaked between 30-60 min. A lack of induction with lyso-PAF, an inactive PAF metabolite, confirmed the specificity of PAF towards this expression. The cells pretreated with CV-6209, a PAF receptor antagonist, did not show any induction of these genes by PAF. It is concluded that PAF causes induction of the early response genes c-fos and TIS-1 in a structurally specific and receptor dependent manner. This finding offers a new role for PAF at the nuclear level and may have important implications in the long term effects of PAF in pathophysiological conditions.

Original languageEnglish (US)
Pages (from-to)1761-1767
Number of pages7
JournalLife Sciences
Volume49
Issue number23
DOIs
StatePublished - Jan 1 1991
Externally publishedYes

Fingerprint

fos Genes
Platelet Activating Factor
Squamous Cell Carcinoma
Genes
Gene Expression
Cycloheximide
Metabolites
Gene expression
Cells
Carcinoma
Messenger RNA

All Science Journal Classification (ASJC) codes

  • Pharmacology

Cite this

Platelet activating factor induces expression of early response genes c-fos and TIS-1 in human epidermoid carcinoma A-431 cells. / Tripathi, Yamini B.; Kandala, Jagannadha C.; Guntaka, Ramareddy; Lim, Robert W.; Shukla, Shivendra D.

In: Life Sciences, Vol. 49, No. 23, 01.01.1991, p. 1761-1767.

Research output: Contribution to journalArticle

Tripathi, Yamini B. ; Kandala, Jagannadha C. ; Guntaka, Ramareddy ; Lim, Robert W. ; Shukla, Shivendra D. / Platelet activating factor induces expression of early response genes c-fos and TIS-1 in human epidermoid carcinoma A-431 cells. In: Life Sciences. 1991 ; Vol. 49, No. 23. pp. 1761-1767.
@article{fe414b4dc92d4feabd29f1616168afd0,
title = "Platelet activating factor induces expression of early response genes c-fos and TIS-1 in human epidermoid carcinoma A-431 cells",
abstract = "The effect of platelet activating factor (PAF) on the induction of early response genes was investigated in A-431 cells (human epidermal carcinoma cells). PAF induced a transient expression of c-fos and TIS-1 mRNA in a time- and dose-dependent manner. As low as 10-10 M PAF caused detectable expression of these genes with a maximum observed at 10-7 M. In the presence of cycloheximide, increases in the gene expression were noticeable at 20 min and peaked between 30-60 min. A lack of induction with lyso-PAF, an inactive PAF metabolite, confirmed the specificity of PAF towards this expression. The cells pretreated with CV-6209, a PAF receptor antagonist, did not show any induction of these genes by PAF. It is concluded that PAF causes induction of the early response genes c-fos and TIS-1 in a structurally specific and receptor dependent manner. This finding offers a new role for PAF at the nuclear level and may have important implications in the long term effects of PAF in pathophysiological conditions.",
author = "Tripathi, {Yamini B.} and Kandala, {Jagannadha C.} and Ramareddy Guntaka and Lim, {Robert W.} and Shukla, {Shivendra D.}",
year = "1991",
month = "1",
day = "1",
doi = "10.1016/0024-3205(91)90319-7",
language = "English (US)",
volume = "49",
pages = "1761--1767",
journal = "Life Sciences",
issn = "0024-3205",
publisher = "Elsevier Inc.",
number = "23",

}

TY - JOUR

T1 - Platelet activating factor induces expression of early response genes c-fos and TIS-1 in human epidermoid carcinoma A-431 cells

AU - Tripathi, Yamini B.

AU - Kandala, Jagannadha C.

AU - Guntaka, Ramareddy

AU - Lim, Robert W.

AU - Shukla, Shivendra D.

PY - 1991/1/1

Y1 - 1991/1/1

N2 - The effect of platelet activating factor (PAF) on the induction of early response genes was investigated in A-431 cells (human epidermal carcinoma cells). PAF induced a transient expression of c-fos and TIS-1 mRNA in a time- and dose-dependent manner. As low as 10-10 M PAF caused detectable expression of these genes with a maximum observed at 10-7 M. In the presence of cycloheximide, increases in the gene expression were noticeable at 20 min and peaked between 30-60 min. A lack of induction with lyso-PAF, an inactive PAF metabolite, confirmed the specificity of PAF towards this expression. The cells pretreated with CV-6209, a PAF receptor antagonist, did not show any induction of these genes by PAF. It is concluded that PAF causes induction of the early response genes c-fos and TIS-1 in a structurally specific and receptor dependent manner. This finding offers a new role for PAF at the nuclear level and may have important implications in the long term effects of PAF in pathophysiological conditions.

AB - The effect of platelet activating factor (PAF) on the induction of early response genes was investigated in A-431 cells (human epidermal carcinoma cells). PAF induced a transient expression of c-fos and TIS-1 mRNA in a time- and dose-dependent manner. As low as 10-10 M PAF caused detectable expression of these genes with a maximum observed at 10-7 M. In the presence of cycloheximide, increases in the gene expression were noticeable at 20 min and peaked between 30-60 min. A lack of induction with lyso-PAF, an inactive PAF metabolite, confirmed the specificity of PAF towards this expression. The cells pretreated with CV-6209, a PAF receptor antagonist, did not show any induction of these genes by PAF. It is concluded that PAF causes induction of the early response genes c-fos and TIS-1 in a structurally specific and receptor dependent manner. This finding offers a new role for PAF at the nuclear level and may have important implications in the long term effects of PAF in pathophysiological conditions.

UR - http://www.scopus.com/inward/record.url?scp=0026093667&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0026093667&partnerID=8YFLogxK

U2 - 10.1016/0024-3205(91)90319-7

DO - 10.1016/0024-3205(91)90319-7

M3 - Article

VL - 49

SP - 1761

EP - 1767

JO - Life Sciences

JF - Life Sciences

SN - 0024-3205

IS - 23

ER -