Pneumocystis carinii infection in germ-free rats: Implications for human patients

Linda Pifer, Charles P. Lattuada, Carol C. Edwards, Diane R. Woods, Dwight R. Owens

Research output: Contribution to journalArticle

19 Citations (Scopus)

Abstract

Pneumocystis carinii infections have been detected both serologically and histologically in untreated, germ-free and conventional rats killed immediately upon arrival from two commercial sources. Pneumocystis antigenemia was detected by counterimmunoelectrophoresis (CIE) and antibody was titered by indirect immunofluorescence. The data suggest that 1) P. carinii is enzootic in certain rat colonies; 2) in utero transmission is a distinct possibility; and 3) paucity of cysts does not rule out P. carinii, as trophozoites predominate in early or subclinical infections. Histologic data support the validity of CIE for noninvasive detection of P. carinii antigen. Infection with this agent may be missed by basing diagnosis upon the presence of cyst forms alone, and it may be more common than previously supposed. These data have significant implications for the natural history, diagnosis, and epidemiology of P. carinii with regard to the human host.

Original languageEnglish (US)
Pages (from-to)23-36
Number of pages14
JournalDiagnostic Microbiology and Infectious Disease
Volume2
Issue number1
DOIs
StatePublished - Jan 1 1984

Fingerprint

Pneumocystis Infections
Pneumocystis carinii
Counterimmunoelectrophoresis
Cysts
Pneumocystis
Trophozoites
Asymptomatic Infections
Indirect Fluorescent Antibody Technique
Natural History
Epidemiology
Antigens
Antibodies
Infection

All Science Journal Classification (ASJC) codes

  • Microbiology (medical)
  • Infectious Diseases

Cite this

Pneumocystis carinii infection in germ-free rats : Implications for human patients. / Pifer, Linda; Lattuada, Charles P.; Edwards, Carol C.; Woods, Diane R.; Owens, Dwight R.

In: Diagnostic Microbiology and Infectious Disease, Vol. 2, No. 1, 01.01.1984, p. 23-36.

Research output: Contribution to journalArticle

Pifer, Linda ; Lattuada, Charles P. ; Edwards, Carol C. ; Woods, Diane R. ; Owens, Dwight R. / Pneumocystis carinii infection in germ-free rats : Implications for human patients. In: Diagnostic Microbiology and Infectious Disease. 1984 ; Vol. 2, No. 1. pp. 23-36.
@article{421b7e6ac3fe43cdaf4e5da789ad2dad,
title = "Pneumocystis carinii infection in germ-free rats: Implications for human patients",
abstract = "Pneumocystis carinii infections have been detected both serologically and histologically in untreated, germ-free and conventional rats killed immediately upon arrival from two commercial sources. Pneumocystis antigenemia was detected by counterimmunoelectrophoresis (CIE) and antibody was titered by indirect immunofluorescence. The data suggest that 1) P. carinii is enzootic in certain rat colonies; 2) in utero transmission is a distinct possibility; and 3) paucity of cysts does not rule out P. carinii, as trophozoites predominate in early or subclinical infections. Histologic data support the validity of CIE for noninvasive detection of P. carinii antigen. Infection with this agent may be missed by basing diagnosis upon the presence of cyst forms alone, and it may be more common than previously supposed. These data have significant implications for the natural history, diagnosis, and epidemiology of P. carinii with regard to the human host.",
author = "Linda Pifer and Lattuada, {Charles P.} and Edwards, {Carol C.} and Woods, {Diane R.} and Owens, {Dwight R.}",
year = "1984",
month = "1",
day = "1",
doi = "10.1016/0732-8893(84)90020-8",
language = "English (US)",
volume = "2",
pages = "23--36",
journal = "Diagnostic Microbiology and Infectious Disease",
issn = "0732-8893",
publisher = "Elsevier Inc.",
number = "1",

}

TY - JOUR

T1 - Pneumocystis carinii infection in germ-free rats

T2 - Implications for human patients

AU - Pifer, Linda

AU - Lattuada, Charles P.

AU - Edwards, Carol C.

AU - Woods, Diane R.

AU - Owens, Dwight R.

PY - 1984/1/1

Y1 - 1984/1/1

N2 - Pneumocystis carinii infections have been detected both serologically and histologically in untreated, germ-free and conventional rats killed immediately upon arrival from two commercial sources. Pneumocystis antigenemia was detected by counterimmunoelectrophoresis (CIE) and antibody was titered by indirect immunofluorescence. The data suggest that 1) P. carinii is enzootic in certain rat colonies; 2) in utero transmission is a distinct possibility; and 3) paucity of cysts does not rule out P. carinii, as trophozoites predominate in early or subclinical infections. Histologic data support the validity of CIE for noninvasive detection of P. carinii antigen. Infection with this agent may be missed by basing diagnosis upon the presence of cyst forms alone, and it may be more common than previously supposed. These data have significant implications for the natural history, diagnosis, and epidemiology of P. carinii with regard to the human host.

AB - Pneumocystis carinii infections have been detected both serologically and histologically in untreated, germ-free and conventional rats killed immediately upon arrival from two commercial sources. Pneumocystis antigenemia was detected by counterimmunoelectrophoresis (CIE) and antibody was titered by indirect immunofluorescence. The data suggest that 1) P. carinii is enzootic in certain rat colonies; 2) in utero transmission is a distinct possibility; and 3) paucity of cysts does not rule out P. carinii, as trophozoites predominate in early or subclinical infections. Histologic data support the validity of CIE for noninvasive detection of P. carinii antigen. Infection with this agent may be missed by basing diagnosis upon the presence of cyst forms alone, and it may be more common than previously supposed. These data have significant implications for the natural history, diagnosis, and epidemiology of P. carinii with regard to the human host.

UR - http://www.scopus.com/inward/record.url?scp=0021719006&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0021719006&partnerID=8YFLogxK

U2 - 10.1016/0732-8893(84)90020-8

DO - 10.1016/0732-8893(84)90020-8

M3 - Article

C2 - 6336148

AN - SCOPUS:0021719006

VL - 2

SP - 23

EP - 36

JO - Diagnostic Microbiology and Infectious Disease

JF - Diagnostic Microbiology and Infectious Disease

SN - 0732-8893

IS - 1

ER -