Point mutation generates constitutive expression of an inducible eukaryotic gene

R. A. Sumrada, Terrance Cooper

Research output: Contribution to journalArticle

21 Citations (Scopus)

Abstract

We describe the analysis of two cis-dominant mutations that result in constitutive expression of the inducible CAR1 gene from yeast. One mutation results from insertion of a Ty element just upstream from the point where CAR1-specific transcription begins. The other mutation is a C-to-G transversion at position -153. Isolation of this point mutation, outside of the transcribed region of CAR1, suggests that expression of this gene is regulated at transcription. It also demonstrates the feasibility and usefulness of analyzing the regulatory sequences of eukaryotic genes on a nucleotide-by-nucleotide basis.

Original languageEnglish (US)
Pages (from-to)643-647
Number of pages5
JournalProceedings of the National Academy of Sciences of the United States of America
Volume82
Issue number3
DOIs
StatePublished - Jan 1 1985

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Point Mutation
Nucleotides
Mutation
Insertional Mutagenesis
Genes
Yeasts
Gene Expression

All Science Journal Classification (ASJC) codes

  • General

Cite this

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AB - We describe the analysis of two cis-dominant mutations that result in constitutive expression of the inducible CAR1 gene from yeast. One mutation results from insertion of a Ty element just upstream from the point where CAR1-specific transcription begins. The other mutation is a C-to-G transversion at position -153. Isolation of this point mutation, outside of the transcribed region of CAR1, suggests that expression of this gene is regulated at transcription. It also demonstrates the feasibility and usefulness of analyzing the regulatory sequences of eukaryotic genes on a nucleotide-by-nucleotide basis.

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