Polypeptide synthesis in alphavirus-infected Aedes albopictus cells during the establishment of persistent infection

M. A. Richardson, R. W. Boulton, Rajendra Raghow, Lynn Dalgarno

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

Polypeptide synthesis was examined in mosquito cells during the establishment of a persistent infection with two alphaviruses, Ross River virus (RRV) and Semliki Forest virus (SFV), and in vertebrate cells cytopathically-infected with the same viruses. In Aedes albopictus cells, RRV reached peak titres at 34-48 hours p.i. At 12 hours 85 per cent of cells assayed as infected by infective centre assay; by 48 hours when persistence was established, virus production was reduced and <5 per cent of cells assayed as infected. There was no shut-down of host polypeptide synthesis during infection. Viral polypeptide synthesis was maximal between 10 and 24 hours p.i. The major viral polypeptides labelled were nucleocapsid protein and envelope protein(s). The precursor polypeptide p95 which was prominent in infected BHK cells was not detected in mosquito cells. Similar results were obtained on SFV infection. During the establishment of persistence there was a coordinate decline in the synthesis of RRV polypeptides, reaching undetectable levels by 72 hours p.i. Subculturing persistently-infected cells led to a small increase in viral polypeptide synthesis and virus titre. In contrast, during RRV growth in BHK cells host protein synthesis was severely inhibited and by 9-11 hours p.i. virus-specific polypeptide synthesis represented more than 90 per cent of total protein synthetic activity.

Original languageEnglish (US)
Pages (from-to)263-274
Number of pages12
JournalArchives of Virology
Volume63
Issue number3-4
DOIs
StatePublished - Sep 1 1980
Externally publishedYes

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Alphavirus
Aedes
Ross River virus
Peptides
Infection
Semliki forest virus
Viruses
Culicidae
Nucleocapsid Proteins
Proteins
Virus Diseases
Viral Load
Vertebrates

All Science Journal Classification (ASJC) codes

  • Virology

Cite this

Polypeptide synthesis in alphavirus-infected Aedes albopictus cells during the establishment of persistent infection. / Richardson, M. A.; Boulton, R. W.; Raghow, Rajendra; Dalgarno, Lynn.

In: Archives of Virology, Vol. 63, No. 3-4, 01.09.1980, p. 263-274.

Research output: Contribution to journalArticle

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