Pontine and basal forebrain cholinergic interaction: Implications for sleep and breathing

Christopher L. Douglas, George J. DeMarco, Helen Baghdoyan, Ralph Lydic

Research output: Contribution to journalArticle

18 Citations (Scopus)

Abstract

Pontine and forebrain cholinergic nuclei contribute to the regulation of breathing and arousal. This report summarizes experiments in rat (n = 20) concerning the cholinergic interaction between pons and basal forebrain. In vitro [ 35S]guanylyl-5′-O-(γ-thio)-triphosphate ([ 35S]GTPγS) autoradiography quantified carbachol-stimulated guanine nucleotide binding (G) protein activation in seven basal forebrain nuclei. Carbachol significantly increased [ 35S]GTPγS binding in the vertical and horizontal limbs of the diagonal band of Broca, medial and lateral septum, and nucleus basalis (B)/substantia innominata (SI). In vitro receptor autoradiography demonstrated muscarinic receptors in the same nuclei where carbachol caused G protein activation. In vivo experiments showed that carbachol administered to the pontine reticular formation (PnO) significantly decreased the number of 7-14 Hz spindles in the electroencephalogram (EEG), decreased acetylcholine release in SI, and decreased respiratory rate. Carbachol microinjection into SI did not alter the number of EEG spindles or respiratory rate. The results help clarify that EEG and rate of breathing are more effectively modulated by cholinergic neurotransmission in PnO than in SI.

Original languageEnglish (US)
Pages (from-to)251-262
Number of pages12
JournalRespiratory Physiology and Neurobiology
Volume143
Issue number2-3
DOIs
StatePublished - Nov 15 2004

Fingerprint

Substantia Innominata
Carbachol
Cholinergic Agents
Sleep
Respiration
Electroencephalography
Septal Nuclei
Respiratory Rate
Autoradiography
GTP-Binding Proteins
Diagonal Band of Broca
Guanine Nucleotides
Pons
Microinjections
Muscarinic Receptors
Prosencephalon
Arousal
Basal Ganglia
Synaptic Transmission
Acetylcholine

All Science Journal Classification (ASJC) codes

  • Neuroscience(all)
  • Physiology
  • Pulmonary and Respiratory Medicine

Cite this

Pontine and basal forebrain cholinergic interaction : Implications for sleep and breathing. / Douglas, Christopher L.; DeMarco, George J.; Baghdoyan, Helen; Lydic, Ralph.

In: Respiratory Physiology and Neurobiology, Vol. 143, No. 2-3, 15.11.2004, p. 251-262.

Research output: Contribution to journalArticle

@article{ccebe50a8493429dba57c2cae52a94ae,
title = "Pontine and basal forebrain cholinergic interaction: Implications for sleep and breathing",
abstract = "Pontine and forebrain cholinergic nuclei contribute to the regulation of breathing and arousal. This report summarizes experiments in rat (n = 20) concerning the cholinergic interaction between pons and basal forebrain. In vitro [ 35S]guanylyl-5′-O-(γ-thio)-triphosphate ([ 35S]GTPγS) autoradiography quantified carbachol-stimulated guanine nucleotide binding (G) protein activation in seven basal forebrain nuclei. Carbachol significantly increased [ 35S]GTPγS binding in the vertical and horizontal limbs of the diagonal band of Broca, medial and lateral septum, and nucleus basalis (B)/substantia innominata (SI). In vitro receptor autoradiography demonstrated muscarinic receptors in the same nuclei where carbachol caused G protein activation. In vivo experiments showed that carbachol administered to the pontine reticular formation (PnO) significantly decreased the number of 7-14 Hz spindles in the electroencephalogram (EEG), decreased acetylcholine release in SI, and decreased respiratory rate. Carbachol microinjection into SI did not alter the number of EEG spindles or respiratory rate. The results help clarify that EEG and rate of breathing are more effectively modulated by cholinergic neurotransmission in PnO than in SI.",
author = "Douglas, {Christopher L.} and DeMarco, {George J.} and Helen Baghdoyan and Ralph Lydic",
year = "2004",
month = "11",
day = "15",
doi = "10.1016/j.resp.2004.02.011",
language = "English (US)",
volume = "143",
pages = "251--262",
journal = "Respiratory Physiology and Neurobiology",
issn = "1569-9048",
publisher = "Elsevier",
number = "2-3",

}

TY - JOUR

T1 - Pontine and basal forebrain cholinergic interaction

T2 - Implications for sleep and breathing

AU - Douglas, Christopher L.

AU - DeMarco, George J.

AU - Baghdoyan, Helen

AU - Lydic, Ralph

PY - 2004/11/15

Y1 - 2004/11/15

N2 - Pontine and forebrain cholinergic nuclei contribute to the regulation of breathing and arousal. This report summarizes experiments in rat (n = 20) concerning the cholinergic interaction between pons and basal forebrain. In vitro [ 35S]guanylyl-5′-O-(γ-thio)-triphosphate ([ 35S]GTPγS) autoradiography quantified carbachol-stimulated guanine nucleotide binding (G) protein activation in seven basal forebrain nuclei. Carbachol significantly increased [ 35S]GTPγS binding in the vertical and horizontal limbs of the diagonal band of Broca, medial and lateral septum, and nucleus basalis (B)/substantia innominata (SI). In vitro receptor autoradiography demonstrated muscarinic receptors in the same nuclei where carbachol caused G protein activation. In vivo experiments showed that carbachol administered to the pontine reticular formation (PnO) significantly decreased the number of 7-14 Hz spindles in the electroencephalogram (EEG), decreased acetylcholine release in SI, and decreased respiratory rate. Carbachol microinjection into SI did not alter the number of EEG spindles or respiratory rate. The results help clarify that EEG and rate of breathing are more effectively modulated by cholinergic neurotransmission in PnO than in SI.

AB - Pontine and forebrain cholinergic nuclei contribute to the regulation of breathing and arousal. This report summarizes experiments in rat (n = 20) concerning the cholinergic interaction between pons and basal forebrain. In vitro [ 35S]guanylyl-5′-O-(γ-thio)-triphosphate ([ 35S]GTPγS) autoradiography quantified carbachol-stimulated guanine nucleotide binding (G) protein activation in seven basal forebrain nuclei. Carbachol significantly increased [ 35S]GTPγS binding in the vertical and horizontal limbs of the diagonal band of Broca, medial and lateral septum, and nucleus basalis (B)/substantia innominata (SI). In vitro receptor autoradiography demonstrated muscarinic receptors in the same nuclei where carbachol caused G protein activation. In vivo experiments showed that carbachol administered to the pontine reticular formation (PnO) significantly decreased the number of 7-14 Hz spindles in the electroencephalogram (EEG), decreased acetylcholine release in SI, and decreased respiratory rate. Carbachol microinjection into SI did not alter the number of EEG spindles or respiratory rate. The results help clarify that EEG and rate of breathing are more effectively modulated by cholinergic neurotransmission in PnO than in SI.

UR - http://www.scopus.com/inward/record.url?scp=7044228168&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=7044228168&partnerID=8YFLogxK

U2 - 10.1016/j.resp.2004.02.011

DO - 10.1016/j.resp.2004.02.011

M3 - Article

C2 - 15519559

AN - SCOPUS:7044228168

VL - 143

SP - 251

EP - 262

JO - Respiratory Physiology and Neurobiology

JF - Respiratory Physiology and Neurobiology

SN - 1569-9048

IS - 2-3

ER -